| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DENN; IG20; RAB3GEP |
| Entrez GeneID | 8567 |
| WB Predicted band size | Calculated MW: 183 kDa; Observed MW: 220 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human DENN |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DENN抗体的3篇代表性文献摘要信息(注：文献标题和作者为虚构示例，仅用于演示格式)：
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1. **文献名称**: *"Characterization of DENND1A Antibodies in Polycystic Ovary Syndrome (PCOS) Studies*"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过制备特异性抗DENND1A抗体，揭示了DENND1A蛋白在多囊卵巢综合征(PCOS)患者卵巢组织中的异常表达，并发现其通过调控Rab35信号通路影响卵泡发育。抗体验证采用Western blot和免疫组化技术。
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2. **文献名称**: *"Role of MADD/DENN in Neuronal Apoptosis: Insights from Antibody-Based Inhibition*"
**作者**: Smith JL, et al.
**摘要**: 利用抗MADD(DENN结构域蛋白)抗体阻断实验，证明MADD通过调控TNF-α受体信号通路参与神经元凋亡过程。研究为神经退行性疾病提供了潜在治疗靶点。
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3. **文献名称**: *"Structural Analysis of DENN Domain Proteins via Cryo-EM and Antibody Mapping*"
**作者**: Brown K, et al.
**摘要**: 通过冷冻电镜结合抗DENN抗体表位定位，解析了DENN结构域蛋白(如DENND2A)的三维构象，揭示了其与Rab GTPases相互作用的分子机制，为开发靶向抑制剂奠定基础。
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**注**：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以关键词“DENN domain antibody”或“DENND1A/MADD antibody”检索真实文献。
DENN antibodies target proteins belonging to the DENN (Differentially Expressed in Normal and Neoplastic cells) domain family, a group of evolutionarily conserved regulators involved in membrane trafficking and Rab GTPase signaling. The DENN domain, typically spanning ~300 amino acids, functions as a guanine nucleotide exchange factor (GEF) for Rab GTPases, which are critical for vesicle formation, transport, and fusion. DENN proteins, including DENND1A-C, DENND2A-D, and DENND3-5. exhibit diverse cellular roles, ranging from clathrin-mediated endocytosis to cytokine secretion and neuronal signaling.
DENN antibodies are essential tools for studying these proteins' expression patterns, subcellular localization, and interactions. Research using DENN antibodies has revealed their involvement in various physiological processes and diseases. For instance, DENND1A is associated with polycystic ovary syndrome (PCOS), while DENND2B mutations link to neurodevelopmental disorders. In cancer, altered DENN protein expression correlates with tumor progression and metastasis, highlighting their potential as therapeutic targets.
The development of DENN antibodies has advanced understanding of Rab-mediated trafficking pathways and their dysregulation in pathologies. Challenges remain in characterizing isoform-specific functions due to structural similarities across DENN family members. Ongoing studies utilize these antibodies to dissect molecular mechanisms in cellular models and validate clinical associations, underscoring their importance in both basic research and translational medicine.
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