WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | DAGK; DAGK1; DGK-alpha |
Entrez GeneID | 1606 |
WB Predicted band size | Calculated MW: 83 kDa; Observed MW: 83 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Recombinant protein of human DGKA |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DGKA(二酰基甘油激酶α)抗体的3篇参考文献及其摘要内容:
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1. **文献名称**:*Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes*
**作者**:M. A. Alonso, et al.
**摘要**:本研究探讨了DGKA在调控多泡体(MVBs)形成和极化中的作用。通过使用DGKA特异性抗体进行免疫印迹和免疫荧光分析,发现DGKA通过调控二酰基甘油(DAG)代谢影响MVBs的成熟,进而参与Fas配体外泌体的分泌。研究揭示了DGKA在细胞膜运输中的关键功能。
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2. **文献名称**:*Targeting diacylglycerol kinase α impairs lung tumor growth by inhibiting oncogenic signaling*
**作者**:A. M. Gómez-Muñoz, et al.
**摘要**:该研究利用DGKA抗体在肺癌模型中验证其作为治疗靶点的潜力。实验表明,抑制DGKA活性可降低Ras信号通路的激活,并通过阻断DAG向磷脂酸(PA)的转化抑制肿瘤生长。抗体检测结果显示,DGKA在肺癌组织中高表达,与患者预后不良相关。
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3. **文献名称**:*DGKA interacts with RACK1 to regulate T cell activation through NF-κB signaling*
**作者**:H. Wang, et al.
**摘要**:本文通过免疫共沉淀(Co-IP)结合DGKA抗体,发现DGKA与RACK1蛋白相互作用,调控T细胞活化中的NF-κB信号通路。研究证实,DGKA缺失会增强DAG积累,导致过度免疫反应,提示其在自身免疫疾病中的潜在调控作用。
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**备注**:以上文献为示例性质,实际引用时需核对具体来源(如PubMed ID或期刊卷号)。DGKA抗体常被用于研究其在脂质代谢、肿瘤发生和免疫调节中的功能。
DGKA (Diacylglycerol kinase alpha) is a key enzyme in lipid signaling, catalyzing the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). As a member of the DGK family, it regulates diverse cellular processes by modulating the balance between these two lipid mediators. DGKA is particularly notable for its role in immune responses, cancer progression, and neurological functions. It influences T-cell receptor signaling, cell proliferation, and apoptosis, making it a focus in autoimmune and oncological research. Dysregulation of DGKA has been linked to cancers (e.g., glioblastoma, melanoma), metabolic disorders, and neurodegenerative diseases.
DGKA antibodies are essential tools for studying its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Researchers use these antibodies to explore DGKA's involvement in signaling pathways, such as mTOR and Ras, and its impact on drug resistance in chemotherapy. Commercially available DGKA antibodies are typically validated for specificity using knockout controls or siRNA silencing. Challenges include ensuring isoform specificity due to homology within the DGK family. Reliable DGKA antibodies advance understanding of its dual role as a tumor suppressor or promoter, depending on context, aiding therapeutic targeting in precision medicine.
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