| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein |
| Entrez GeneID | 1616 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Daxx |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及DAXX抗体的典型文献摘要，供参考：
1. **文献名称**："Role of the histone H3.3 chaperone DAXX in apoptosis and viral infection"
**作者**：T. K. Kim et al.
**摘要**：研究通过DAXX抗体验证了DAXX蛋白在组蛋白H3.3沉积中的作用，并发现其通过调控染色质结构影响细胞凋亡和抗病毒反应，尤其在疱疹病毒感染中DAXX与病毒蛋白相互作用并抑制复制。
2. **文献名称**："DAXX regulates mitotic progression and DNA damage response in cancer cells"
**作者**：L. Wang et al.
**摘要**：利用DAXX特异性抗体进行免疫荧光和Western blot分析，发现DAXX通过调控Aurora B激酶活性维持有丝分裂保真性，其缺失导致染色体不稳定并增强肿瘤细胞对DNA损伤药物的敏感性。
3. **文献名称**："PML/DAXX-dependent regulation of TGF-β signaling"
**作者**：S. S. Lin et al.
**摘要**：通过免疫共沉淀(使用DAXX抗体)证实DAXX与PML核体协同作用，抑制TGF-β信号通路中的Smad2/3磷酸化，揭示了其在癌症转移中的潜在调控机制。
4. **文献名称**："DAXX interacts with ATRX and modulates telomere maintenance"
**作者**：J. R. Woodgett et al.
**摘要**：研究使用DAXX抗体进行ChIP-seq分析，发现DAXX与ATRX形成复合物，共同维持端粒异染色质结构，其突变与胶质瘤中ALT(替代性端粒延长)机制激活相关。
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**备注**：以上文献为虚拟示例，实际引用需根据具体研究需求检索PubMed或Google Scholar，推荐结合关键词“DAXX antibody application”或“DAXX protein function”筛选高引论文。
The Daxx (Death domain-associated protein 6) antibody is a key tool for studying the multifunctional Daxx protein, which plays roles in apoptosis, transcriptional regulation, and chromatin remodeling. Daxx primarily localizes to promyelocytic leukemia nuclear bodies (PML-NBs) and interacts with PML, p53. and other proteins involved in stress response pathways. It acts as a scaffold protein, modulating apoptosis through FAS and ASK1 signaling, while also serving as a histone chaperone for the H3.3 variant during chromatin assembly.
Daxx antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its nuclear dynamics, interactions with partners like ATRX (a chromatin remodeler), and involvement in diseases. Dysregulation of Daxx expression or mutations in its binding partners are linked to cancers, neurodevelopmental disorders, and autoimmune conditions. For example, Daxx/ATRX complexes are critical for maintaining heterochromatin and telomere integrity, with mutations observed in gliomas and pancreatic neuroendocrine tumors.
Commercial Daxx antibodies are often validated for specificity using knockout cell lines or siRNA-mediated depletion. Researchers prioritize clones that recognize conserved epitopes across species (human, mouse, rat) to enable cross-model studies. Its dual roles in pro-apoptotic and anti-apoptotic contexts, depending on cellular context, make Daxx a compelling target for both basic and translational research.
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