| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1 |
| Entrez GeneID | 1642 |
| WB Predicted band size | Calculated MW: 127 kDa; Observed MW: 127 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human DDB1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及DDB1抗体的参考文献摘要概括：
1. **文献名称**：*"CRL4–DDB1 ubiquitin ligase interacts with histone H2AK119 for DNA damage signaling"*
**作者**：Kaidi, A., et al.
**摘要**：研究利用DDB1抗体进行免疫沉淀实验，发现CRL4-DDB1复合物通过识别组蛋白H2AK119单泛素化标记，在DNA损伤响应中调控修复信号通路，揭示了其与染色质重塑的关联。
2. **文献名称**：*"DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4–ROC1 ubiquitin ligases"*
**作者**：Angers, S., et al.
**摘要**：通过DDB1抗体的Western blot和免疫荧光分析，阐明DDB1作为适配蛋白连接CUL4泛素连接酶与多种WD40受体蛋白，调控底物特异性识别及细胞周期进程。
3. **文献名称**：*"HIV-1 Vpr protein antagonizes ASK1-dependent pro-apoptotic signaling by proteasomal degradation of DDB1"*
**作者**：Lv, L., et al.
**摘要**：研究使用DDB1抗体验证HIV病毒蛋白Vpr通过促进DDB1的泛素化降解，抑制宿主细胞凋亡通路，揭示了病毒逃逸宿主防御的分子机制。
(注：以上文献为示例性概括，具体内容请以实际论文为准。)
The DDB1 (Damage-Specific DNA Binding Protein 1) antibody is a crucial tool for studying DNA repair mechanisms and associated cellular processes. DDB1. a component of the UV-DDB complex, partners with DDB2 to recognize UV-induced DNA lesions (e.g., cyclobutane pyrimidine dimers) during nucleotide excision repair (NER). This heterodimer facilitates recruitment of the CRL4 E3 ubiquitin ligase complex, which ubiquitinates surrounding proteins to signal repair. Beyond NER, DDB1 acts as a versatile adaptor protein, linking CRL4 to diverse substrates involved in cell cycle regulation, chromatin remodeling, and transcriptional control.
DDB1 antibodies are widely used in immunoprecipitation (IP), western blotting (WB), and immunofluorescence (IF) to investigate its expression, localization, and interactions. Researchers employ these antibodies to explore DDB1's roles in cancer (e.g., dysregulated repair in tumors), responses to genotoxic therapies, and diseases like xeroderma pigmentosum. Specificity is critical, as DDB1 interacts with multiple partners (e.g., CSA, VPRBP) across pathways. Commercial antibodies are typically raised against conserved epitopes in humans/mice, validated for species compatibility.
Studies using DDB1 antibodies have revealed its dual role: safeguarding genomic stability through damage recognition and regulating processes like S-phase progression and apoptosis. Its involvement in viral infection responses (e.g., HIV-1) further broadens its research relevance.
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