Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

大包装询价

货号: IPD-E04493h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的ANG-Ⅱ含量。ANG-Ⅱ即血管紧张素Ⅱ，在心血管系统调节中有着重要背景。它可引起血管收缩、醛固酮释放等。研究机制方面，其通过与特异性受体结合，激活多条信号通路，影响细胞生长、增殖，在高血压、心衰等疾病发病中扮演关键角色。试剂盒检测范围为31.25 pg/ml-2000 pg/ml.，适用于科研场景中探究ANG - Ⅱ在心血管疾病模型中的动态变化、药物干预效果评估或相关代谢调控机制分析，为心血管疾病基础研究、药理学实验及生物标志物探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
angiotensin Ⅱ, angiotensin 2, ANG2
缩写：
ANG-Ⅱ
种属：
Homo sapiens (Human)
样本类型：
serum, plasma
检测范围：
31.25 pg/ml-2000 pg/ml.
灵敏度：
15.6 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ANG- Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-101
1:2	Average %	89
	Range %	82-95
1:4	Average %	93
	Range %	88-99
1:8	Average %	96
	Range %	89-103

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average
2000	0.159	0.154	0.157
1000	0.214	0.222	0.218
500	0.292	0.286	0.289
250	0.370	0.398	0.384
125	0.568	0.561	0.565
62.5	0.915	0.933	0.924
31.25	1.346	1.284	1.315
0	2.207	2.125	2.166

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANG-II antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ANG-II antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.