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产品描述：人细胞纤维连接蛋白(cFn)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的cFn含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，尤其适用于细胞生物学研究、组织再生机制探索以及心血管疾病、纤维化疾病模型中基质蛋白的定量分析；为体外研究细胞 - 基质相互作用、药物干预效果评估及病理机制解析提供可靠工具，满足科研领域对细胞外基质蛋白的精准检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CIG ELISA Kit; Cold insoluble globulin ELISA Kit; Cold-insoluble globulin ELISA Kit; DKFZp686F10164 ELISA Kit; DKFZp686H0342 ELISA Kit; DKFZp686I1370 ELISA Kit; DKFZp686O13149 ELISA Kit; ED B ELISA Kit; Fibronectin 1 ELISA Kit; FINC ELISA Kit; FINC_HUMAN ELISA Kit; FN ELISA Kit; FN1 ELISA Kit; FNZ ELISA Kit; GFND ELISA Kit; GFND2 ELISA Kit; LETS ELISA Kit; Migration stimulating factor ELISA Kit; MSF ELISA Kit; Ugl-Y3 ELISA Kit
缩写：cFn
Uniprot No.：P02751
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cFn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 88-100 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human cFn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 8002.1192.1262.1232.010 4001.7971.7041.7511.638 2001.2741.2851.2801.167 1000.7580.7610.7600.647 500.4820.4950.4890.376 250.2860.2960.2910.178 12.50.1960.2030.2000.087 00.1120.1130.113 | ||||||
货期：3-5 working days
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