| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PPP3CA; CALNA; CNA; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform |
| Entrez GeneID | 5530 |
| WB Predicted band size | Calculated MW: 59 kDa; Observed MW: 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Calcineurin A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Calcineurin A抗体的3篇参考文献及其简要摘要：
1. **"Calcineurin: From Structure to Function"**
- **作者**: Klee, C.B., et al.
- **摘要**: 该综述详细描述了钙调磷酸酶(Calcineurin)的分子结构，重点分析了其催化亚基Calcineurin A(PPP3CA)的功能域及调控机制。文中提到利用特异性抗体研究其亚细胞定位及与钙调蛋白的相互作用，为后续信号通路研究奠定基础。
2. **"Immunolocalization of Calcineurin A in Cardiac Hypertrophy Models"**
- **作者**: Taigen, T., et al.
- **摘要**: 通过制备高特异性Calcineurin A抗体，研究团队在心肌肥大小鼠模型中观察到该蛋白的表达上调，并证实其与NFAT信号通路的激活直接相关。抗体被用于Western blot和免疫组化验证组织特异性表达。
3. **"Calcineurin A Antibody-Based Detection in Neurodegenerative Diseases"**
- **作者**: Wu, H.Y., et al.
- **摘要**: 文章开发了一种单克隆抗体，用于检测阿尔茨海默病脑组织中Calcineurin A的异常磷酸化状态。实验证明该抗体可特异性识别病理状态下Calcineurin A的构象变化，提示其作为疾病生物标志物的潜力。
如需更多文献或特定研究方向的参考文献，可进一步补充说明。
Calcineurin A (CnA) is the catalytic subunit of calcineurin, a calcium/calmodulin-dependent serine/threonine protein phosphatase. It plays a critical role in calcium-mediated signaling pathways, particularly in immune response, neuronal development, and cardiac function. Calcineurin A exists in three isoforms (α, β, γ) encoded by distinct genes (PPP3CA, PPP3CB, PPP3CC), with CnAα and CnAβ being widely expressed in tissues like the brain, muscle, and immune cells, while CnAγ is testis-specific.
Antibodies targeting Calcineurin A are essential tools for studying its expression, localization, and activity in physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate calcineurin's role in T-cell activation (via NFAT signaling), synaptic plasticity, and hypertrophy. Dysregulation of calcineurin is implicated in diseases like cardiac hypertrophy, neurodegenerative disorders, and autoimmune conditions.
These antibodies often recognize conserved regions across species (human, mouse, rat) and require validation for isoform specificity, as cross-reactivity can occur. Researchers also utilize phospho-specific antibodies to study post-translational modifications regulating calcineurin activity. Proper controls, such as knockout validation, are recommended to ensure antibody reliability in experimental models.
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