首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管活性肠肽(VIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VIP含量。VIP即血管活性肠肽，是一种神经递质和激素。在它分布广泛，参与多种生理调节。研究机制上，VIP通过与特定受体结合，激活细胞内信号通路，在神经调节、免疫调节、血管舒张等过程中发挥重要作用，对其研究有助于深入了解相关生理病理机制。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可借助开展VIP在消化系统疾病、神经免疫调节及代谢相关研究中的表达水平检测，例如探究肠易激综合征、神经退行性疾病或免疫微环境调控的分子机制，为体外实验模型、动物组织或体液样本的VIP定量分析提供可靠工具，适用于基础医学研究、药效评价及生物标志物探索等非诊断用途的科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Peptide histidine methioninamide 27 ELISA Kit; Peptide histidine valine 42 ELISA Kit; PHM27 ELISA Kit; PHV42 ELISA Kit; Prepro VIP ELISA Kit; Vasoactive intestinal peptide ELISA Kit; Vasoactive intestinal polypeptide ELISA Kit; VIP ELISA Kit; VIP peptides ELISA Kit; VIP peptides precursor ELISA Kit; VIP_HUMAN ELISA Kit
缩写：VIP
Uniprot No.：P01282
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 89-105 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7952.5982.6972.5475002.3202.1892.2552.1052501.6441.5641.6041.4541250.9130.8850.8990.74962.50.4250.4160.4210.27131.250.2890.2810.2850.13515.60.2270.2240.2260.07600.1510.1490.150 | ||||||
货期：3-5 working days
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