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产品描述：人羧化不全基质Gla蛋白(ucMGP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ucMGP含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于心血管疾病机制研究、抗钙化药物疗效评估、慢性肾病进展监测等科研领域，尤其满足实验室对微量样本的高通量检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cell growth inhibiting gene 36 protein ELISA Kit; Cell growth-inhibiting gene 36 protein ELISA Kit; GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX ELISA Kit; GIG36 ELISA Kit; Matrix Gla protein ELISA Kit; MGLAP ELISA Kit; MGP ELISA Kit; MGP_HUMAN ELISA Kit; NTI ELISA Kit
缩写：ucMGP
Uniprot No.：P08493
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ucMGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1000 | Average % | 90 | |
| Range % | 85-98 | ||
| 1:2000 | Average % | 97 | |
| Range % | 91-105 | ||
| 1:4000 | Average % | 92 | |
| Range % | 85-99 | ||
| 1:8000 | Average % | 93 | |
| Range % | 86-97 |
回收率：
| The recovery of human ucMGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-102 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.0072.0172.0121.903 51.5261.5361.5311.422 2.51.0971.1191.1080.999 1.250.6350.6890.6620.553 0.6250.4290.4650.4470.338 0.3120.2640.2870.2760.167 0.1560.1900.1860.1880.079 00.1100.1080.109 | ||||||
货期：3-5 working days
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