首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人松弛肽/松弛素(RLN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RLN1含量。人松弛肽/松弛素（RLN）是一种多功能的肽激素，主要通过与特定的受体结合发挥作用。RLN在生殖、心血管适应、应激反应、代谢控制等方面具有重要作用，其作用机制涉及调节细胞增殖、迁移、凋亡和细胞外基质重塑等过程。试剂盒检测范围为6.25 pg/mL-400 pg/mL，可为科研领域研究RLN在生理调控机制、生殖相关疾病模型或心血管代谢关联实验中的表达变化提供可靠工具；适用于细胞实验、动物模型或临床前研究中的蛋白定量分析，为探索RLN的生物学功能及相关分子机制提供精准的科研数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：H1 ELISA Kit; H1RLX ELISA Kit; Preprorelaxin H1 ELISA Kit; Prorelaxin ELISA Kit; Prorelaxin H1 ELISA Kit; REL1_HUMAN ELISA Kit; Relaxin A chain ELISA Kit; Relaxin H1 ELISA Kit; RLN 1 ELISA Kit; Rln1 ELISA Kit; RLXH1 ELISA Kit
缩写：RLN1
Uniprot No.：P04808
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human relaxin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 83-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human relaxin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 87 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4001.8881.9641.9261.7982001.5191.4251.4721.3441000.9960.9860.9910.863500.6340.6440.6390.511250.3820.3940.3880.26012.50.2970.2860.2920.1646.250.2010.2060.2040.07600.1270.1280.128 | ||||||
货期：3-5 working days
×
