首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肾上腺髓质素2(ADM2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的ADM2含量。人肾上腺髓质素2（ADM2）是一种小分子活性肽，作为心血管、肾脏、肺脏等重要脏器的内源性保护因子，参与机体内环境稳态的调节，具有降低血压、减轻炎症、保护心肌和肺功能等作用。研究显示，ADM2通过IGF-II信号通路修复血脑屏障，对焦虑障碍及相关精神疾病的治疗提供新的潜在靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于科研领域中对ADM2表达调控机制的探索，如代谢性疾病动物模型的血浆检测、体外细胞实验中分泌水平的动态监测，或药物干预下ADM2生物标志物变化的评估，为心血管及代谢相关的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADM2 ELISA Kit; ADM2_HUMAN ELISA Kit; Adrenomedullin 2 ELISA Kit; AM2 ELISA Kit; dJ579N16.4 ELISA Kit; FLJ21135 ELISA Kit; IMDL ELISA Kit; IMDS ELISA Kit; Intermedin ELISA Kit; Intermedin-long ELISA Kit; Intermedin-short ELISA Kit
缩写：ADM2
Uniprot No.：Q7Z4H4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of human ADM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 97-107 | ||||
| EDTA plasma (n=4) | 92 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.9883.0363.0122.87410002.3652.4882.4272.2895001.6091.6751.6421.5042500.9010.9090.9050.7671250.5450.5250.5350.39762.50.3750.3890.3820.24431.250.2780.2580.2680.13000.1350.1400.138 | ||||||
货期：3-5 working days
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