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产品描述：人抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AVP含量。AVP即精氨酸加压素，在人体生理中起重要作用。它参与调节水盐平衡、血管收缩等。研究上，聚焦其在相关受体的作用机制，探索其与疾病如尿崩症、心血管病等关联，旨在开发针对AVP靶点的药物以治疗相关病症。试剂盒检测范围为1.25 pg/mL-80 pg/mL，科研人员可将其应用于内分泌调控研究、心血管疾病动物模型评估或水盐代谢相关实验，特别适用于探究下丘脑 - 垂体轴功能、渗透压调节机制以及病理状态下体液失衡的分子基础，为神经内分泌领域的体外实验研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AVP ELISA Kit; ARVP ELISA Kit; VPVasopressin-neurophysin 2-copeptin ELISA Kit; AVP-NPII) [Cleaved into: Arg-vasopressin ELISA Kit; Arginine-vasopressin); Neurophysin 2 ELISA Kit; Neurophysin-II); Copeptin] ELISA Kit
缩写：AVP
Uniprot No.：P01185
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.312 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 83 | ||||
| Range % | 81-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 85-98 | ||||
| EDTA plasma (n=4) | 94 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected802.5672.5022.5352.393401.8511.8161.8341.692201.3011.2431.2721.130100.8520.8090.8310.68950.5320.5140.5230.3812.50.3830.3760.3800.2381.250.2470.2390.2430.10100.1450.1390.142 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AVP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AVP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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