首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体片断3b(C3b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、脑脊液（CSF）、唾液样本中的C3b含量。C3b是补体系统中的重要分子。其背景在于补体系统是免疫防御的关键部分。研究机制方面，C3b参与调理吞噬作用，能与病原体结合促进吞噬细胞识别吞噬；还参与补体激活的旁路途径，在免疫调控、炎症反应等过程中发挥着重要作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，本产品适用于基础医学研究中对补体系统活性的动态监测，例如探究感染性疾病、神经炎症或慢性炎症模型中C3b的生物学功能，也可用于评估药物或生物制剂对补体活化途径的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C3b
种属：Homo sapiens (Human)
样本类型：serum, urine, cerebrospinal fluid (CSF), saliva
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 87 | |||||||||||
| Range % | 82-92 | ||||||||||||
| 1:2 | Average % | 98 | |||||||||||
| Range % | 92-103 | ||||||||||||
| 1:4 | Average % | 99 | |||||||||||
| Range % | 93-103 | ||||||||||||
| 1:8 | Average % | 94 | |||||||||||
| Range % | 89-98 | ||||||||||||
回收率：
| The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 95 | 90-98 | |||||||||||
| EDTA plasma (n=4) | 97 | 91-99 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected2002.1152.0032.0591.9321001.7481.7361.7421.615501.2451.2661.2561.129250.7560.7820.7690.64212.50.5680.5720.5700.4436.250.3330.3420.3380.2113.120.1990.1870.1930.06600.1280.1260.127 | |||||||||||||
货期：3-5 working days
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