| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK 3; JTK5; JTK5A; Ryk; RYK1; Vik |
| Entrez GeneID | 6259 |
| WB Predicted band size | Calculated MW: 68 kDa; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human RYK |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RYK抗体的3-4篇文献概览(内容基于虚构，仅供参考)：
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1. **文献名称**：*Characterization of a Novel Monoclonal Antibody Against the RYK Receptor Tyrosine Kinase*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种新型单克隆抗体，用于特异性识别RYK受体胞外结构域。通过Western blot和免疫荧光验证其在多种哺乳动物细胞系中的反应性，证实该抗体适用于检测RYK蛋白的表达及定位，为研究RYK在Wnt信号通路中的作用提供工具。
2. **文献名称**：*RYK Expression in Neural Development: Insights from Immunohistochemical Analysis*
**作者**：Lee J, et al.
**摘要**：利用商业化RYK多克隆抗体，研究者分析了小鼠胚胎神经系统发育过程中RYK的时空表达模式。结果显示RYK在神经管闭合和轴突导向中显著富集，提示其可能参与神经发育调控。
3. **文献名称**：*RYK as a Potential Biomarker in Colorectal Cancer: Validation of Antibody Specificity*
**作者**：Chen X, et al.
**摘要**：研究评估了两种抗RYK抗体的特异性(兔源多抗和小鼠单抗)，发现单抗在结直肠癌组织中的信号更稳定。抗体通过siRNA敲降实验验证，确认其与RYK蛋白的特异性结合，支持RYK作为癌症预后标志物的潜力。
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注：以上文献为示例，实际引用请通过PubMed、Google Scholar等平台检索关键词“RYK antibody”或“RYK receptor characterization”获取真实文献。
RYK (Related to receptor tyrosine kinase) is a unique transmembrane receptor belonging to the atypical receptor tyrosine kinase (RTK) family. Unlike conventional RTKs, RYK lacks intrinsic kinase activity due to evolutionary mutations in its kinase domain. Initially identified in *Drosophila* as *Derailed*, it plays critical roles in developmental processes, including axon guidance, neural crest cell migration, and organogenesis. In mammals, RYK functions primarily as a co-receptor in Wnt signaling pathways, particularly interacting with Wnt5a to regulate planar cell polarity (PCP) and β-catenin-independent pathways. It associates with Frizzled receptors to mediate non-canonical Wnt signaling, influencing cell migration, polarity, and tissue patterning.
RYK’s extracellular domain contains a Wnt-binding WIF domain, while its intracellular region facilitates interactions with downstream effectors like Dishevelled. Dysregulation of RYK is implicated in developmental defects, cancer (e.g., leukemia, breast cancer, glioblastoma), and neurological disorders. For instance, aberrant RYK expression correlates with tumor invasiveness and poor prognosis.
RYK-specific antibodies are essential tools for studying its expression, localization, and signaling mechanisms. These antibodies target distinct epitopes (e.g., extracellular or intracellular domains) and are validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence. Commercial RYK antibodies are available from suppliers like Santa Cruz Biotechnology and Abcam, often accompanied by knockdown/knockout validation to ensure specificity. Research on RYK continues to uncover its therapeutic potential in targeting Wnt-related pathologies.
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