| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-binding protein SATB1 (Special AT-rich sequence-binding protein 1) |
| Entrez GeneID | 6304 |
| WB Predicted band size | Calculated MW: 86 kDa; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human SATB1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与SATB1抗体相关的代表性文献(虚拟示例，实际文献请通过学术数据库检索)：
1. **"SATB1 regulates chromatin organization in breast cancer metastasis"**
- Alvarez et al., 2012
- 研究发现SATB1通过染色质重构调控乳腺癌转移基因网络，使用SATB1抗体进行ChIP-seq分析揭示了其与基因组特定区域的结合模式。
2. **"Antibody-based profiling of SATB1 in T-cell development"**
- Liu & Watanabe, 2008
- 开发了特异性SATB1单克隆抗体，通过免疫荧光和流式细胞术证实SATB1在胸腺T细胞分化中的动态表达变化。
3. **"SATB1 as a prognostic marker in colorectal cancer"**
- Zhang et al., 2015
- 采用SATB1抗体进行免疫组化分析，发现高表达SATB1与结直肠癌患者生存率降低显著相关，提示其作为临床预后标志物的潜力。
4. **"SATB1 antibody validation for chromatin interaction studies"**
- Kumar et al., 2019
- 系统验证了多种SATB1抗体的特异性，比较了Western blot、免疫沉淀等实验中的性能差异，为染色质构象研究提供抗体选择指南。
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**提示**：实际研究中建议通过PubMed或Web of Science搜索最新文献，关键词组合如"SATB1 antibody validation"或"SATB1 immunohistochemistry"。部分关键研究可能涉及抗体在表观遗传调控、癌症或免疫研究中的具体应用场景。
SATB1 (Special AT-rich sequence-binding protein 1) is a chromatin-organizing protein that plays a critical role in regulating gene expression by modulating higher-order chromatin architecture. It binds to AT-rich DNA sequences, acting as a global transcriptional regulator that coordinates the spatial organization of chromatin loops. SATB1 is particularly important in T-cell development, where it governs thymocyte differentiation and the expression of immune-related genes. Beyond immunology, it has been implicated in cancer progression, with studies linking its overexpression to metastatic potential in breast, colorectal, and other cancers.
SATB1 antibodies are essential tools for studying its functional dynamics. These antibodies typically target specific epitopes, such as the N-terminal PDZ-like domain or C-terminal homeodomain, enabling applications like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence. Researchers use SATB1 antibodies to investigate its role in chromatin remodeling, transcriptional networks, and disease mechanisms. Commercial SATB1 antibodies are validated for species cross-reactivity (human, mouse, rat) and assay compatibility. Recent work explores SATB1's diagnostic potential, as its expression patterns correlate with clinical outcomes in certain malignancies. However, antibody validation remains crucial due to SATB1's multiple isoforms and post-translational modifications that may affect detection accuracy.
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