首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体1q(C1q)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1QB含量。C1QB是补体系统C1复合物的重要组成部分，补体系统在免疫防御等过程中发挥关键作用。相关研究主要围绕其在补体激活机制中的功能展开，探究其与免疫相关疾病的关联、在信号传导中的作用等，旨在为疾病治疗提供新靶点和思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域中C1q相关的免疫调控机制研究、疾病模型构建或药物干预效果评估等场景，例如通过检测实验动物血清或组织中的C1q浓度，探究其在炎症性疾病或神经退行性疾病中的分子作用机制，为相关基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1qb ELISA Kit; C1QB_HUMAN ELISA Kit; Complement C1q subcomponent subunit B ELISA Kit; Complement component 1 q subcomponent B chain ELISA Kit; Complement component 1 q subcomponent beta polypeptide ELISA Kit; Complement component C1q B chain ELISA Kit; Complement subcomponent C1q chain B ELISA Kit
缩写：C1QB
Uniprot No.：P02746
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1q in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 100-106 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 92-106 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of human C1q spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 102-108 | |||||
| EDTA plasma (n=4) | 95 | 90-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.0151.9521.9841.85951.7581.8781.8181.6932.51.5141.5691.5421.4171.250.9950.9680.9820.8570.6250.6140.6320.6230.4980.3120.4750.4570.4660.3410.1560.3340.3550.3450.22000.1230.1260.125 | |||||||
货期：3-5 working days
×
