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产品描述：人鞭毛蛋白(flagellin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的flagellin含量。鞭毛蛋白（flagellin）是细菌鞭毛的主要组成部分。它能被宿主模式识别受体识别，激活先天性免疫反应。相关研究聚焦于其与免疫细胞的相互作用机制，探索将其作为免疫佐剂、疫苗抗原等，以增强免疫效果、防治感染等疾病。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品广泛应用于科研领域，如肠道炎症性疾病（如克罗恩病、溃疡性结肠炎）的机制研究、细菌感染模型中宿主 - 病原体相互作用评估，以及肠道微生物群与免疫调节相关性等基础研究，为探索鞭毛蛋白在先天免疫中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：flagellin
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human flagellin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-104 | |||||
回收率：
| The recovery of human flagellin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-93 | ||||
| EDTA plasma (n=4) | 95 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.9552.9602.9582.804252.5772.5052.5412.38712.51.6861.7991.7431.5896.250.8490.8880.8690.7153.120.4310.4110.4210.2671.560.2230.2420.2330.0790.780.2010.2050.2030.04900.1550.1520.154 | ||||||
货期：3-5 working days
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