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产品描述：人α1防御素 (DEFA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的DEFA1含量。DEFA1 是一种防御素，存在于人体中性粒细胞中。它在机体天然免疫防御中发挥重要作用，对抵御病原体入侵至关重要。研究机制方面，它能与病原体细胞膜相互作用，破坏膜结构，还参与免疫调节，影响免疫细胞的活性和功能。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于基础科研领域，如感染性疾病机制研究、免疫调节功能探索、炎症相关动物模型验证，以及药物开发过程中生物标志物的动态监测，为研究DEFA1在宿主防御系统中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：alpha 1 ELISA Kit; DEF1 ELISA Kit; DEF1_HUMAN ELISA Kit; DEFA1 ELISA Kit; DEFA1B ELISA Kit; DEFA2 ELISA Kit; Defensin 1 ELISA Kit; Defensin ELISA Kit; Defensin; alpha 1 ELISA Kit; Defensin; alpha 1; myeloid related sequence ELISA Kit; Defensin; alpha 2 ELISA Kit; HNP-1 ELISA Kit; HNP-2 ELISA Kit; HNP1 ELISA Kit; HP-1 ELISA Kit; HP-2 ELISA Kit; HP1 ELISA Kit; HP2 ELISA Kit; MRS ELISA Kit; Myeloid related sequence ELISA Kit; Neutrophil defensin 1 ELISA Kit; Neutrophil defensin 2 ELISA Kit
缩写：DEFA1
Uniprot No.：P59665
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of human DEFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-102 | ||||
| EDTA plasma (n=4) | 90 | 86-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.0582.0142.0361.933201.5471.5251.5361.433101.1041.0711.0880.98550.6730.7150.6940.5912.50.4590.4370.4480.3451.250.3090.3120.3110.2080.6250.2050.1940.2000.09700.1070.0990.103 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human DEFA1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled DEFA1antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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