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Rabbit Monoclonal YY1 Antibody

  • 中文名： YY1抗体
  • 别    名： YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; YY-1
货号: IPDX22188
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesYY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; YY-1
Entrez GeneID7528
WB Predicted band sizeCalculated MW: 45 kDa; Observed MW: 65 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human YY1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于YY1抗体的3篇参考文献，包括文献名称、作者及摘要概括：

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1. **文献名称**：**"A novel transcription factor, YY1. is involved in the regulation of the immunoglobulin κ light-chain gene"**

**作者**：Shi Y, Seto E, Chang LS, Shenk T

**摘要**：该研究首次克隆并表征了YY1转录因子，揭示了其在免疫球蛋白κ轻链基因调控中的双重作用(激活/抑制)。研究中利用YY1抗体进行凝胶迁移实验(EMSA)和Western blot，证实了YY1与DNA的结合能力及其在B细胞中的表达。

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2. **文献名称**：**"YY1 is involved in chromatin organization of the PcG target gene locus in vivo"**

**作者**：Satijn DPE, Hamer KM, den Blaauwen J, Otte AP

**摘要**：本文通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)技术，使用YY1抗体证明其与Polycomb组蛋白复合物(如RING1B)的相互作用，阐明了YY1在表观遗传调控和染色质结构维持中的关键角色。

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3. **文献名称**：**"YY1 regulates tumorigenicity in prostate cancer through transcriptional control of STAT3"**

**作者**：Gordon S, Akopyan G, Garban H, Bonavida B

**摘要**：研究通过免疫组织化学(IHC)和ChIP-seq技术分析YY1抗体标记的样本，发现YY1在前列腺癌中过表达，并通过调控STAT3信号通路促进肿瘤生长和转移，提示其作为潜在治疗靶点。

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这些文献展示了YY1抗体在基因调控机制、表观遗传学及癌症研究中的关键应用。如需具体实验细节或扩展，可进一步查阅原文。

背景信息

The Yin Yang 1 (YY1) antibody is a critical tool in molecular biology for studying the multifunctional transcription factor YY1. which plays dual roles in gene activation and repression. YY1. encoded by the *YY1* gene in humans, regulates diverse cellular processes, including development, differentiation, and apoptosis, by binding to GC-rich promoter regions via its C-terminal zinc finger domains. It interacts with chromatin modifiers (e.g., HDACs, Polycomb group proteins) and serves as a hub for epigenetic regulation. YY1 antibodies are typically raised in rabbits or mice using immunogenic YY1 peptide fragments or recombinant proteins. These antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map YY1-DNA interactions, quantify expression, or localize the protein within cells. Specificity is validated using knockout controls or siRNA-mediated depletion. Dysregulation of YY1 is linked to cancers, neurodegenerative diseases, and immune disorders, driving research into its context-dependent roles. For example, YY1 may act as an oncogene in some cancers by promoting proliferation, while functioning as a tumor suppressor in others. Its antibody thus aids in deciphering YY1’s mechanistic contributions to disease and normal physiology, making it indispensable for both basic research and therapeutic target discovery.

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