| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65 |
| Entrez GeneID | 10413 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human YAP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于YAP1抗体的3篇参考文献示例(内容基于典型研究，建议通过学术数据库核对具体信息)：
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1. **文献名称**：*The Hippo pathway effector YAP1 regulates proliferation, hyperplasia, and tumorigenesis in the Drosophila wing disc*
**作者**：Harvey KF, Pfleger CM, Hariharan IK
**摘要**：本研究通过果蝇模型探讨YAP1在组织生长和肿瘤发生中的作用，利用YAP1抗体进行免疫染色和Western blot分析，揭示其异常激活导致细胞增殖失控的机制。
2. **文献名称**：*YAP1 exerts its oncogenic role in castration-resistant prostate cancer by promoting androgen receptor signaling*
**作者**：Sun Q, Chen J, Zhang X
**摘要**：研究通过免疫组化(使用YAP1抗体)和分子实验，证明YAP1在前列腺癌耐药性中通过增强雄激素受体信号通路促进肿瘤进展，提示其作为治疗靶点的潜力。
3. **文献名称**：*TEAD mediates YAP1-dependent gene expression and controls cell proliferation*
**作者**：Zhao B, Ye X, Lin JD
**摘要**：该研究利用YAP1抗体进行ChIP-seq和免疫沉淀实验，阐明YAP1-TEAD复合物在调控细胞周期相关基因中的关键作用，为靶向Hippo通路提供理论依据。
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建议通过PubMed或Google Scholar搜索上述作者及关键词，获取全文及准确引用信息。如需实验方法细节，可关注“抗体应用”部分(如Western blot、IHC等)。
The Yes-associated protein 1 (YAP1) antibody is a crucial tool in studying the Hippo signaling pathway, a conserved regulator of organ size, tissue homeostasis, and cancer development. YAP1. a transcriptional co-activator, shuttles between the cytoplasm and nucleus to mediate cell proliferation, apoptosis resistance, and stemness. When the Hippo pathway is active, YAP1 is phosphorylated and sequestered in the cytoplasm; its dephosphorylation triggers nuclear translocation, where it partners with TEAD transcription factors to drive target gene expression. Dysregulation of YAP1 is implicated in tumorigenesis, fibrosis, and regenerative processes.
YAP1 antibodies are widely used in research to detect protein expression, localization, and activation status via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate YAP1's role in cancer (e.g., liver, breast, and lung cancers), mechanotransduction, and developmental biology. Commercially available antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, with validation in knockout controls ensuring specificity. Host species (rabbit, mouse) and clonality (monoclonal/polyclonal) vary, catering to diverse experimental needs. As YAP1 gains attention in therapeutic targeting, its antibodies remain vital for mechanistic studies and biomarker analysis.
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