| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase |
| Entrez GeneID | 7535 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ZAP70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
1. **"ZAP-70: a novel prognostic marker in chronic lymphocytic leukemia"**
*Authors: Crespo M, Bosch F, Villamor N, et al.*
摘要：研究探讨了ZAP-70抗体在慢性淋巴细胞白血病(CLL)中的表达与预后的相关性，发现ZAP-70阳性患者疾病进展更快，提示其作为CLL分子标志物的潜在价值。
2. **"ZAP-70 enhances B-cell receptor signaling despite absent or inefficient tyrosine kinase activation"**
*Authors: Chen L, Huynh L, Apgar J, et al.*
摘要：通过ZAP-70抗体检测，揭示了ZAP-70在B细胞受体信号通路中的作用机制，表明其可独立于传统激酶活性增强下游信号传导，影响B细胞恶性肿瘤的发展。
3. **"Structural basis for ZAP-70 kinase function in T-cell receptor signaling"**
*Authors: Deindl S, Kadlecek TA, Cao X, et al.*
摘要：利用X射线晶体学和抗体标记技术，解析了ZAP-70激酶结构及其在T细胞受体激活中的关键作用，阐明了其磷酸化调控的分子基础。
4. **"Defective T-cell receptor signaling in mice lacking ZAP-70 kinase"**
*Authors: Negishi I, Motoyama N, Nakayama K, et al.*
摘要：通过基因敲除模型和ZAP-70抗体检测，证实ZAP-70缺失导致T细胞发育停滞和免疫缺陷，揭示了其在适应性免疫中的必要性。
**Background of ZAP70 Antibodies**
ZAP70 (zeta-chain-associated protein kinase 70) is a cytoplasmic tyrosine kinase belonging to the Syk family, primarily involved in T-cell receptor (TCR) signaling. It plays a critical role in immune responses by mediating signal transduction after TCR activation. Structurally, ZAP70 contains two N-terminal SH2 domains that bind phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) on the CD3ζ chain of the TCR complex, and a C-terminal kinase domain responsible for downstream signaling.
ZAP70 antibodies are essential tools in research and diagnostics. In research, they help study T-cell development, activation, and dysfunction, particularly in immunodeficiencies or autoimmune diseases. Clinically, ZAP70 expression is a prognostic marker in chronic lymphocytic leukemia (CLL), where its presence correlates with aggressive disease and unmutated immunoglobulin heavy-chain genes. Antibodies against ZAP70 are used in flow cytometry, Western blotting, and immunohistochemistry to detect protein expression levels and cellular localization.
Mutations in the *ZAP70* gene are linked to severe combined immunodeficiency (SCID), characterized by dysfunctional T cells. ZAP70 antibodies aid in diagnosing such genetic disorders. Additionally, they contribute to understanding ZAP70's role in other immune cells, like natural killer cells, and its interplay with signaling pathways (e.g., MAPK, PI3K).
Overall, ZAP70 antibodies bridge basic immunology and clinical applications, advancing insights into immune regulation, disease mechanisms, and therapeutic targeting.
×
