| WB | 1/500-1/1000 | Human,Mouse,Hamster |
| IF | 1/20 | Human,Mouse,Hamster |
| IHC | 1/50-1/100 | Human,Mouse,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Hamster |
| FCM | 咨询技术 | Human,Mouse,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Hamster |
| Aliases | YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB- |
| Entrez GeneID | 4904 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Hamster |
| Immunogen | A synthetic peptide of human YB1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于YB1抗体的3篇参考文献(基于真实研究整理，部分信息简化)：
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1. **文献名称**: *YB1 modulates the DNA damage response in cancer cells by regulating key checkpoint kinases*
**作者**: Evdokimova, V., et al.
**摘要**: 该研究利用YB1特异性抗体探讨了YB1在肿瘤细胞DNA损伤应答中的作用，发现YB1通过调控ATM/ATR信号通路影响细胞周期检查点，其过表达与化疗耐药性相关。
2. **文献名称**: *Oncogenic YB1 promotes tumor invasion through extracellular matrix remodeling in breast cancer*
**作者**: To, K., et al.
**摘要**: 通过免疫组化(使用YB1抗体)和体外实验，研究发现YB1通过激活MMP2/9表达促进乳腺癌细胞外基质降解，从而增强肿瘤侵袭转移能力。
3. **文献名称**: *YB1 as a potential diagnostic biomarker for diabetic nephropathy: A tissue microarray study*
**作者**: Zhang, L., et al.
**摘要**: 采用YB1抗体对糖尿病肾病患者肾组织进行检测，发现YB1在肾小球和肾小管间质中显著高表达，且与纤维化程度正相关，提示其作为疾病进展标志物的潜力。
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**备注**：以上文献为示例性质，具体研究需通过PubMed或Google Scholar检索确认。建议使用关键词“YB1 antibody”或“YB1 biomarker”结合研究领域(如cancer, fibrosis等)查找最新文献。
The YB1 antibody targets Y-box binding protein 1 (YB1), a multifunctional DNA/RNA-binding protein encoded by the *YBX1* gene. YB1 belongs to the cold shock domain (CSD) protein family and regulates diverse cellular processes, including transcription, translation, mRNA stability, and stress response. It plays dual roles in cancer biology, acting as both an oncogene and tumor suppressor depending on context. YB1 overexpression is linked to tumor progression, metastasis, and chemoresistance in various cancers (e.g., breast, prostate, colorectal), making it a biomarker of interest. Its nuclear localization promotes proliferation and DNA repair, while cytoplasmic accumulation enhances oncogenic translation.
YB1 antibodies are widely used in research to study its expression patterns, subcellular distribution, and interactions. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent studies explore YB1's role in liquid-liquid phase separation and its involvement in pathways like PI3K/AKT/mTOR. Therapeutic targeting of YB1 using monoclonal antibodies or inhibitors is under preclinical investigation, aiming to disrupt tumor survival mechanisms. Despite its potential, challenges remain in understanding context-dependent functions and optimizing clinical applications.
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