| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YES1; YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes |
| Entrez GeneID | 7525 |
| WB Predicted band size | Calculated MW: 61 kDa; Observed MW: 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Yes1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Yes1抗体的3篇参考文献示例，内容基于模拟数据整理：
1. **文献名称**: "Yes1 Kinase as a Therapeutic Target in Triple-Negative Breast Cancer"
**作者**: Smith A, et al.
**摘要**: 研究通过Yes1特异性抗体证实Yes1在TNBC细胞中高表达，抑制Yes1活性可降低肿瘤侵袭性，提示其作为治疗靶点的潜力。
2. **文献名称**: "Role of Yes1 in EGFR Signaling Pathway Regulation"
**作者**: Lee J, et al.
**摘要**: 利用Yes1抗体进行免疫共沉淀实验，发现Yes1与EGFR在肺癌细胞中存在直接互作，调控下游MAPK通路激活，影响细胞增殖。
3. **文献名称**: "Yes1 Antibody-Based Detection of Tumor Metastasis Markers"
**作者**: Chen R, et al.
**摘要**: 开发高灵敏度Yes1单克隆抗体，通过免疫组化验证其在结直肠癌组织中的过表达，且与淋巴结转移呈正相关，可用于临床预后评估。
4. **文献名称**: "Structural Insights into Yes1 Kinase Inhibition by Small Molecules"
**作者**: Gonzalez M, et al.
**摘要**: 结合Yes1抗体结晶学分析和体外激酶实验，揭示Yes1 ATP结合域构象变化，为设计选择性抑制剂提供结构基础。
(注：以上文献信息为示例性内容，实际引用需以真实发表的学术论文为准。)
**Background of Yes1 Antibody**
The Yes1 antibody is a crucial tool in studying Yes-associated protein 1 (YAP1), a downstream effector of the Hippo signaling pathway. YAP1. encoded by the *YES1* gene, regulates cell proliferation, apoptosis, and organ size by interacting with transcription factors like TEAD. Dysregulation of YAP1 is linked to cancers, fibrosis, and developmental disorders due to its role in promoting cell growth and survival.
Yes1 antibodies are designed to detect and quantify YAP1 expression in tissues or cells, enabling research into its activation, localization, and interactions. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). In cancer research, Yes1 antibodies help assess YAP1 overexpression or nuclear accumulation, which correlates with tumor progression, metastasis, and poor prognosis.
Recent studies explore YAP1's potential as a therapeutic target, driving interest in Yes1 antibodies for preclinical validation of inhibitors. Additionally, they aid in understanding YAP1's crosstalk with other pathways (e.g., Wnt, EGFR) and its role in stem cell maintenance. Commercial Yes1 antibodies vary in specificity, requiring validation via knockout controls to ensure reliability. Overall, Yes1 antibodies remain vital for unraveling YAP1's complex roles in health and disease.
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