| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | alpha pal; alpha palindromic binding protein; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; NRF-1; Nrf1; NRF1_HUMAN; Nuclear respiratory factor 1; transcription factor 11. |
| Entrez GeneID | 4899 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human NRF1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Nrf1抗体的3篇参考文献及其摘要概括：
1. **"Nrf1 is essential for the transcriptional induction of glutathione biosynthesis and oxidative stress responses"**
- **作者**: Zhang Y. et al.
- **摘要**: 本研究通过Western blot和免疫荧光技术，利用Nrf1抗体验证其在细胞氧化应激中的作用，发现Nrf1通过调控谷胱甘肽合成关键基因的表达维持细胞氧化还原平衡。
2. **"Nrf1 regulates cellular resistance to cisplatin through modulating the antioxidant response"**
- **作者**: Lee J. et al.
- **摘要**: 文章通过免疫组化和Western blot分析，发现Nrf1抗体检测到其在癌细胞中表达上调，并通过激活抗氧化通路增强对化疗药物顺铂的耐受性。
3. **"Nrf1-mediated mitochondrial proteostasis: Implications in neurodegenerative diseases"**
- **作者**: Hirotsu Y. et al.
- **摘要**: 研究使用Nrf1抗体结合ChIP-seq技术，揭示Nrf1通过调控线粒体蛋白稳态相关基因，在帕金森病模型中延缓神经退行性病变的分子机制。
4. **"A novel role of Nrf1 in regulating hepatic lipid metabolism via PPARα"**
- **作者**: Wang H. et al.
- **摘要**: 通过免疫沉淀和免疫印迹实验，结合Nrf1抗体，证明Nrf1与PPARα相互作用调控肝脏脂代谢，为代谢综合征治疗提供新靶点。
(注：上述文献为示例，实际引用需根据具体研究内容检索PubMed或Google Scholar获取。)
Nrf1 (Nuclear factor erythroid 2-related factor 1), also known as NFE2L1. is a transcription factor belonging to the CNC-bZIP family. It plays a critical role in regulating cellular responses to oxidative stress, mitochondrial function, and proteasome homeostasis. Unlike its homolog Nrf2. which primarily activates antioxidant response element (ARE)-driven genes under stress, Nrf1 is constitutively expressed and essential for basal oxidative stress management. It is synthesized as an endoplasmic reticulum (ER)-anchored protein, undergoing complex post-translational processing (e.g., glycosylation, proteolytic cleavage) to become an active nuclear transcription factor. Nrf1 regulates genes involved in glutathione synthesis, phase II detoxification enzymes, and proteasome subunits, maintaining redox balance and protein quality control. Its dysfunction has been linked to neurodegenerative diseases, metabolic disorders, and cancer.
Nrf1 antibodies are essential tools for studying its expression, localization, and molecular mechanisms. They enable detection of Nrf1 isoforms (e.g., p120. p95. p85) via Western blot, immunofluorescence, or chromatin immunoprecipitation (ChIP). Commercially available antibodies often target specific domains (e.g., N-terminal, C-terminal), but researchers must validate specificity due to potential cross-reactivity with Nrf2 or other family members. Applications include investigating Nrf1's role in mitochondrial biogenesis, ER stress responses, and its crosstalk with pathways like mTOR and UPR. Proper controls (e.g., knockout cells, peptide blocking) are critical for reliable data interpretation.
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