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产品描述：人14-3-3 蛋白 beta/alpha (YWHAB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的YWHAB含量。YWHAB是一种重要靶点，它在细胞信号传导等生理过程中发挥关键作用。研究机制方面，聚焦于其与多种信号通路的相互作用，探索其在肿瘤发生发展等疾病进程里的角色。深入研究YWHAB或可为相关疾病的治疗提供新策略。试剂盒检测范围为6.25 ng/mL-400 ng/mL，其宽泛的线性范围和多样化的样本兼容性，特别适用于基础科研中探索YWHAB在疾病模型中的动态变化、信号通路机制研究或药物干预效果评价等场景，为揭示该蛋白在代谢调控、细胞增殖等领域的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14 3 3 ELISA Kit; 14 3 3 protein beta ELISA Kit; 14 3 3 protein beta/alpha ELISA Kit; 14 3 3 protein zeta ELISA Kit; 14 3 3 zeta ELISA Kit; 14-3-3 protein beta/alpha ELISA Kit; 14-3-3 protein/cytosolic phospholipase A2 ELISA Kit; 1433B_HUMAN ELISA Kit; GW128 ELISA Kit; HS1 ELISA Kit; KCIP 1 ELISA Kit; KCIP-1 ELISA Kit; MGC111427 ELISA Kit; MGC126532 ELISA Kit; MGC138156 ELISA Kit; N-terminally processed ELISA Kit; Protein 1054 ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; delta polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; zeta polypeptide ELISA Kit; YWHAB ELISA Kit; YWHAD ELISA Kit; YWHAZ ELISA Kit
缩写：YWHAB
Uniprot No.：P31946
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human YWHAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-95 | ||||
| EDTA plasma (n=4) | 97 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.5522.6022.5772.4562001.7491.7971.7731.6521001.0281.0831.0560.935500.5690.5430.5560.435250.3380.3160.3270.20612.50.2460.2400.2430.1226.250.1670.1610.1640.04300.1250.1160.121 | ||||||
货期：3-5 working days
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