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产品描述:人核酸酶-敏感元件结合蛋白1(YBX1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的YBX1含量。YBX1是一个关键靶点。它在细胞增殖、凋亡、代谢等多种生理过程中发挥作用。研究发现,YBX1异常表达与肿瘤发生、发展及耐药性密切相关。其机制涉及调控相关基因转录、翻译等,为肿瘤等疾病研究和治疗提供了新方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,该产品适用于基础科研领域,如肿瘤微环境研究、基因表达调控机制探索、细胞信号转导分析等方向,为研究者提供可靠的YBX1蛋白定量工具,助力于分子病理学、细胞功能学及相关药物开发等实验场景的数据获取。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:BP 8 ELISA Kit; CBF-A ELISA Kit; CCAAT binding transcription factor I subunit A ELISA Kit; CCAAT-binding transcription factor I subunit A ELISA Kit; CSDA2 ELISA Kit; CSDB ELISA Kit; DBPB ELISA Kit; DNA binding protein B ELISA Kit; DNA-binding protein B ELISA Kit; EFI-A ELISA Kit; Enhancer factor I subunit A ELISA Kit; MDR NF1 ELISA Kit; MGC104858 ELISA Kit; MGC110976 ELISA Kit; MGC117250 ELISA Kit; NSEP 1 ELISA Kit; NSEP1 ELISA Kit; Nuclease sensitive element binding protein 1 ELISA Kit; Nuclease-sensitive element-binding protein 1 ELISA Kit; p50 ELISA Kit; Q15905 ELISA Kit; Y-box binding protein 1 ELISA Kit; Y-box transcription factor ELISA Kit; Y-box-binding protein 1 ELISA Kit; YB 1 ELISA Kit; YB-1 ELISA Kit; YBOX1_HUMAN ELISA Kit; YBX 1 ELISA Kit; ybx1 ELISA Kit
缩写:YBX1
Uniprot No.:P67809
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.8 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human YBX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 89 | |||||
Range % | 81-93 | ||||||
1:2 | Average % | 102 | |||||
Range % | 95-109 | ||||||
1:4 | Average % | 95 | |||||
Range % | 91-99 | ||||||
1:8 | Average % | 86 | |||||
Range % | 83-91 |
回收率:
The recovery of human YBX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 104 | 101-109 | |||||
EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected20002.6582.6982.6782.57510002.3812.4512.4162.3135001.9741.9621.9681.8652501.2291.1691.1991.0961250.6980.6870.6930.59062.50.4000.4120.4060.30331.250.2090.2110.2100.10700.1010.1050.103 |
货期:3-5 working days
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