| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NR1D1; EAR1; HREV; THRAL; Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1 |
| Entrez GeneID | 9572 |
| WB Predicted band size | Calculated MW: 67 kDa; Observed MW: 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human NR1D1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Rev-erbα regulates hepatic lipid metabolism through repression of lipoprotein lipase gene expression"**
*作者：Le Martelot et al.*
摘要：该研究利用NR1D1抗体通过染色质免疫沉淀(ChIP)和Western blot技术，揭示Rev-erbα通过抑制脂蛋白脂肪酶基因调控肝脏脂代谢的机制。
2. **"Circadian clock control by SUMOylation of BMAL1"**
*作者：Cardone et al.*
摘要：文献通过NR1D1抗体进行免疫共沉淀实验，证明BMAL1的SUMO化修饰影响生物钟核心蛋白(包括Rev-erbα)的节律性表达及功能。
3. **"Nuclear receptor Rev-erbα is a critical lithium-sensitive component of the circadian clock"**
*作者：Yin et al.*
摘要：研究使用NR1D1抗体在小鼠脑组织中进行免疫组化分析，发现Rev-erbα作为锂盐敏感靶点，调控生物钟基因Per2的周期性表达。
4. **"Anti-inflammatory effects of Rev-erbα via transcriptional repression of pro-inflammatory genes"**
*作者：Sato et al.*
摘要：通过NR1D1抗体进行ChIP-seq和荧光素酶报告实验，证实Rev-erbα通过直接结合炎症相关基因启动子区域，抑制巨噬细胞炎症反应。
Nuclear receptor subfamily 1 group D member 1 (NR1D1), also known as REV-ERBα, is a ligand-regulated transcription factor belonging to the nuclear receptor superfamily. It plays a critical role in regulating circadian rhythms, metabolic homeostasis, and inflammatory responses. NR1D1 functions as a transcriptional repressor by binding to ROR response elements (RORE) in target gene promoters, competing with ROR family activators. It interacts with corepressors like NCoR/HDAC3 to suppress genes involved in lipid metabolism, glucose regulation, and clock-controlled pathways. Dysregulation of NR1D1 has been linked to metabolic disorders, circadian disruption, and cancer progression.
NR1D1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). High-quality antibodies specifically recognize epitopes in the ligand-binding or DNA-binding domains, allowing differentiation between isoforms or post-translationally modified forms. Validation typically includes knockout cell line controls and functional assays. Researchers use these antibodies to investigate NR1D1's tissue-specific roles, diurnal expression patterns, and therapeutic potential in metabolic syndrome or circadian-related diseases. Commercial NR1D1 antibodies are commonly raised in rabbits or mice, with monoclonal versions offering enhanced specificity for quantitative studies.
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