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产品描述：人Xin肌动蛋白结合含重复序列蛋白(XIRP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的XIRP1含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于基础科研中XIRP1的功能机制研究，例如心血管疾病模型中的蛋白表达动态分析、肿瘤微环境下的细胞迁移能力评估，或药物干预实验中分子标记物的定量追踪，为体外实验提供可靠的蛋白水平检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI415219 ELISA Kit; Cardiomyopathy-associated protein 1 ELISA Kit; CMYA1 ELISA Kit; DKFZp451D042 ELISA Kit; DKFZp779C1255 ELISA Kit; DKFZp779C1947 ELISA Kit; MGC144145 ELISA Kit; MGC144146 ELISA Kit; Xin actin-binding repeat-containing protein 1 ELISA Kit; XIN ELISA Kit; Xirp1 ELISA Kit; XIRP1_HUMAN ELISA Kit
缩写：XIRP1
Uniprot No.：Q702N8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human XIRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 90-104 | |||||
回收率：
| The recovery of human XIRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 93-96 | ||||
| EDTA plasma (n=4) | 101 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20001.9171.9471.9321.75010001.2861.2991.2931.1115000.8040.8150.8100.6282500.5360.5050.5210.3391250.4010.4350.4180.23662.50.3120.3230.3180.13631.250.2470.2610.2540.07200.1810.1830.182 | ||||||
货期：3-5 working days
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