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Mouse Protein Wnt-7a(WNT7A) ELISA kit

  • 中文名: 小鼠Wnt-7a蛋白(WNT7A)ELISA kit
货号: IPD-EL026141MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠Wnt-7a蛋白(WNT7A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WNT7A含量。WNT7A是一种参与神经发生、血管生成和血脑屏障维持的重要蛋白。其研究机制涉及通过调节细胞周期、神经元分化和突触形成等过程,对神经发生的多个步骤进行关键控制。此外,WNT7A在疾病如神经退行性疾病、癌症和角膜损伤修复中发挥重要作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL,适用于基础研究中对Wnt - 7a功能的探索,如发育生物学中组织特异性表达分析、骨骼疾病或神经系统病变机制研究,以及靶向Wnt通路的药物干预效果评估。其兼容性设计支持多种生物样本的并行检测,为科研人员在细胞模型、动物模型及离体组织实验中定量追踪Wnt - 7a动态变化提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Wnt7a ELISA Kit; Wnt-7a ELISA Kit; Protein Wnt-7a ELISA Kit
  • 缩写:
    WNT7A
  • Uniprot No.:
    P24383
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Stem Cells
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    93


    Range %

    86-100


    1:2

    Average %

    98


    Range %

    91-105


    1:4

    Average %

    94


    Range %

    89-100


    1:8

    Average %

    102


    Range %

    97-106


  • 回收率:

    The recovery of mouse WNT7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    85-98


    EDTA plasma (n=4)

    96

    90-102


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    3.163

    3.014

    3.088

    2.979


    5

    2.308

    2.267

    2.288

    2.178


    2.5

    1.554

    1.528

    1.541

    1.431


    1.25

    0.928

    0.919

    0.923

    0.814


    0.625

    0.613

    0.629

    0.621

    0.511


    0.312

    0.403

    0.393

    0.398

    0.288


    0.156

    0.287

    0.284

    0.285

    0.176


    0

    0.111

    0.108

    0.110




  • 货期:
    3-5 working days

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