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产品描述：小鼠Wnt-5a蛋白(WNT5A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的WNT5A含量。WNT5A是一种与肿瘤发生和发展密切相关的基因，其既可以促癌也可以抑癌，其信号级联反应复杂，受多种因子调节。WNT5A在细胞增殖、凋亡、周期等生物学功能中发挥作用，其研究机制涉及多种信号通路和细胞过程。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于探索不同生理病理条件下Wnt-5a的动态表达特征，如细胞分化模型中的信号通路分析、基因编辑动物模型的表型验证或药物干预实验中的生物标志物检测。配套提供标准品、检测抗体及显色试剂，支持实验室常规开展细胞生物学、发育生物学及疾病机制相关的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt5a ELISA Kit; Wnt-5a ELISA Kit; Protein Wnt-5a ELISA Kit
缩写：WNT5A
Uniprot No.：P22725
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of mouse WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-98 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.7552.6522.7042.5361002.3242.3762.3502.182501.6391.7521.6961.528250.9541.0350.9950.82712.50.5650.5240.5450.3776.250.3720.3590.3660.1983.120.2570.2590.2580.09000.1710.1650.168 | ||||||
货期：3-5 working days
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