| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NC; APUG; NERCC; LCCS10; NERCC1 |
| Entrez GeneID | 91754 |
| WB Predicted band size | Calculated MW: 107 kDa; Observed MW: 107 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human NEK9 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NEK9抗体的3篇文献摘要信息，供参考：
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1. **文献名称**：*NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA*
**作者**：Yamada et al.
**摘要**：该研究揭示NEK9通过调控自噬过程参与纤毛形成，利用特异性抗体证实NEK9与肌球蛋白IIA(MYH9)相互作用，并证明其缺失导致纤毛生成异常，提示NEK9在细胞骨架动态和纤毛相关疾病中的作用。
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2. **文献名称**：*NEK9 kinase is involved in mitotic checkpoint control through phosphorylation of CDC20 and MAD2*
**作者**：Roig et al.
**摘要**：研究发现NEK9通过磷酸化有丝分裂检查点蛋白CDC20和MAD2调控细胞周期进程。使用NEK9抗体进行免疫沉淀和Western blot分析，发现其功能异常导致染色体分离错误，与癌症基因组不稳定性相关。
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3. **文献名称**：*Structural and functional analysis of the human NEK9 kinase: insights into oligomerization and activation*
**作者**：Meek et al.
**摘要**：通过晶体结构和抗体介导的功能实验，解析NEK9激酶寡聚化及激活机制，发现其C端卷曲螺旋结构域对自磷酸化及下游信号传导至关重要，为靶向NEK9的药物开发提供结构基础。
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(注：以上文献信息为示例性概括，实际引用请以具体论文内容为准。)
NEK9 (NIMA-related kinase 9) is a serine/threonine kinase belonging to the NEK family, which plays critical roles in cell cycle regulation, particularly during mitosis. It is involved in key processes such as centrosome maturation, spindle assembly, and cytokinesis. NEK9 interacts with other NEK family members (e.g., NEK6. NEK7) and regulatory proteins like HURP and the RanGTPase system to coordinate mitotic progression. Its activation is tightly regulated through phosphorylation cascades, often triggered by cell cycle checkpoints. Dysregulation of NEK9 has been linked to mitotic errors, chromosomal instability, and diseases including cancer and developmental disorders.
NEK9 antibodies are essential tools for studying its expression, localization, and function in cellular models. These antibodies are typically developed against specific epitopes, such as the kinase domain or regulatory regions, and validated for applications like Western blotting, immunofluorescence, and immunoprecipitation. Researchers use NEK9 antibodies to investigate its role in cell division mechanisms, DNA damage responses, and interactions with signaling pathways (e.g., PLK1. Aurora kinases). Commercial NEK9 antibodies often undergo specificity testing using knockout cell lines to ensure reliability. Understanding NEK9's molecular mechanisms via antibody-based assays contributes to insights into mitotic regulation and potential therapeutic targets for cancer treatment.
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