WB | 1/500-1/1000 | Mouse,Rat |
IF | 1/20 | Mouse,Rat |
IHC | 咨询技术 | Mouse,Rat |
ICC | 技术咨询 | Mouse,Rat |
FCM | 咨询技术 | Mouse,Rat |
Elisa | 咨询技术 | Mouse,Rat |
Aliases | NEK7; NimA related protein kinase 7 |
Entrez GeneID | 140609 |
WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 35 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Mouse,Rat |
Immunogen | A synthetic peptide of human NEK7 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是与NEK7抗体相关的3篇文献示例(信息为模拟生成,仅供参考):
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1. **文献名称**:*NEK7 regulates mitosis and cell death in hepatocellular carcinoma through Aurora kinase A*
**作者**:Li X, et al.
**摘要**:研究发现NEK7在肝癌细胞中通过磷酸化Aurora激酶A调控有丝分裂进程,NEK7抗体被用于检测其在肿瘤组织中的表达水平,揭示其高表达与患者预后不良相关。
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2. **文献名称**:*NEK7-dependent NLRP3 inflammasome activation drives obesity-associated inflammation*
**作者**:He Y, et al.
**摘要**:文章阐明NEK7在肥胖模型中通过结合NLRP3炎症小体促进IL-1β分泌,使用NEK7特异性抗体阻断该互作可减轻小鼠代谢性炎症,提示其作为治疗靶点的潜力。
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3. **文献名称**:*A structural perspective on the regulation of NEK7-kinase activity in cell division*
**作者**:Bertran MT, et al.
**摘要**:通过冷冻电镜技术解析NEK7蛋白结构,结合抗体表位定位实验,揭示NEK7在纺锤体组装中的构象变化机制,为靶向NEK7的抑制剂开发提供依据。
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如需实际文献,建议通过PubMed或Google Scholar以“NEK7 antibody”为关键词检索近年论文。
NEK7 (NIMA-related kinase 7) is a serine/threonine kinase belonging to the NEK family, which plays crucial roles in cell cycle regulation, particularly in mitotic progression. It is essential for the G2/M transition and spindle assembly, acting as a key mediator in the separation of chromosomes during mitosis. NEK7 interacts with components of the NLRP3 inflammasome, linking it to inflammatory responses and diseases like autoinflammatory disorders. Dysregulation of NEK7 has been implicated in cancer, as abnormal expression correlates with tumorigenesis, metastasis, and chemoresistance in various cancers, including breast and colorectal cancers.
NEK7 antibodies are widely used in research to study its expression, localization, and function in cellular processes. These antibodies enable techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect NEK7 in human, mouse, or rat tissues and cell lines. Specific monoclonal or polyclonal antibodies target distinct epitopes, aiding in elucidating NEK7's role in mitosis, inflammasome activation, and disease mechanisms. Validated NEK7 antibodies are critical for ensuring experimental accuracy, particularly in exploring its dual roles in cell division and inflammation. Commercial antibodies are available from suppliers like Abcam and CST, often accompanied by knockdown/knockout validation data to confirm specificity. Research on NEK7 continues to expand, highlighting its potential as a therapeutic target in cancer and inflammatory conditions.
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