| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NRK2; STK2; pp12301 |
| Entrez GeneID | 6787 |
| WB Predicted band size | Calculated MW: 95 kDa; Observed MW: 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NEK4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于NEK4抗体的3篇示例参考文献(注：以下内容为模拟示例，非真实存在的文献)：
---
1. **文献名称**: *NEK4 regulates ATR-mediated DNA damage checkpoint through phosphorylation of Chk1*
**作者**: Smith J, et al. (2018)
**摘要**: 本研究使用NEK4特异性抗体进行Western blot和免疫沉淀实验，发现NEK4通过磷酸化Chk1调控ATR介导的DNA损伤应答。抗体验证显示NEK4在HeLa细胞中高表达，并在电离辐射后与ATR形成复合物。
---
2. **文献名称**: *NEK4 localizes to the basal body of primary cilia and its loss promotes cystic kidney disease*
**作者**: Brown T, et al. (2015)
**摘要**: 通过免疫荧光染色(使用兔源NEK4多克隆抗体)，作者证明NEK4定位于纤毛基部，并发现NEK4缺失导致小鼠模型多囊肾表型。抗体特异性通过siRNA敲低和质谱分析验证。
---
3. **文献名称**: *Development of a NEK4-selective inhibitor for anti-cancer therapy*
**作者**: Lee S, et al. (2022)
**摘要**: 利用NEK4单克隆抗体进行免疫组化分析，发现NEK4在非小细胞肺癌组织中高表达。研究者开发了靶向NEK4的小分子抑制剂，并通过抗体依赖性激酶活性检测验证其抑制效果。
---
**注**：以上文献为示例，实际引用时请核查PubMed等数据库获取真实文献。如需具体文章，可补充研究背景进一步筛选。
NEK4 (NIMA-related kinase 4) is a serine/threonine kinase belonging to the NEK family, which is evolutionarily conserved and involved in cell cycle regulation, particularly mitotic progression. First identified in mammals through homology to the Aspergillus nidulans NIMA kinase, NEK4 has been implicated in diverse cellular processes, including centrosome maturation, microtubule dynamics, DNA damage response, and cellular stress signaling. Unlike other NEK kinases with well-characterized mitotic roles, NEK4 exhibits distinct subcellular localization patterns, primarily associating with the cytoplasm and mitochondria, suggesting functional specialization.
Research indicates NEK4 interacts with proteins like HSP90 and PUM2. influencing cell survival under stress conditions. Its dysregulation has been linked to pathological states, particularly cancer, where it may act as either an oncogene or tumor suppressor depending on context. Elevated NEK4 expression correlates with poor prognosis in certain cancers (e.g., hepatocellular carcinoma), while loss of function is observed in others (e.g., gliomas).
NEK4 antibodies are critical tools for investigating these mechanisms, enabling detection of protein expression levels, phosphorylation status, and subcellular localization through techniques like Western blotting, immunofluorescence, and immunoprecipitation. Commercial antibodies typically target specific epitopes in the kinase domain or C-terminal regulatory regions. However, validation remains essential due to potential cross-reactivity with other NEK family members. Ongoing studies using NEK4 antibodies aim to clarify its dual roles in tumorigenesis and explore therapeutic targeting opportunities.
×
