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产品描述：人Wnt-5b蛋白(WNT5B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的WNT5B含量。WNT5B是一个靶点。它是Wnt蛋白家族成员，在胚胎发育、细胞迁移与极性调控等多方面发挥关键作用。其研究机制涉及与受体结合激活非经典Wnt信号通路，异常表达与肿瘤、炎症等疾病相关，在临床研究中受重点关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研领域对WNT5B蛋白表达水平的动态监测，例如癌症机制研究、再生医学模型构建或信号通路交互作用分析。科研人员可通过该产品在体外实验中探索WNT5B在疾病模型中的功能，或评估药物干预对相关信号通路的调控效应，为分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MGC2648 ELISA Kit; Protein Wnt-5b ELISA Kit; Wingless type MMTV integration site family, member 5B ELISA Kit; WNT 5B ELISA Kit; WNT 5B protein ELISA Kit; Wnt family member 5B ELISA Kit; Wnt5b ELISA Kit; WNT5B protein ELISA Kit; WNT5B_HUMAN ELISA Kit
缩写：WNT5B
Uniprot No.：Q9H1J7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-89 | |||||
回收率：
| The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6632.7672.7152.61652.3262.2252.2762.1772.51.6751.5781.6271.5281.251.0851.0221.0540.9550.6250.5070.5230.5150.4160.3120.2640.2760.2700.1710.1560.1780.1870.1830.08400.0990.0980.099 | ||||||
货期：3-5 working days
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