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产品描述：人Wnt-5a蛋白(WNT5A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT5A含量。WNT5A是一个重要靶点，属于Wnt蛋白家族。它参与多条信号通路调节，在细胞增殖、分化、迁移等过程中发挥关键作用。异常表达与肿瘤发生发展、胚胎发育异常等密切相关。目前围绕其作用机制及靶向治疗开展了诸多研究。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础科研领域，如肿瘤微环境研究、干细胞分化机制探索、炎症相关信号通路分析等场景，可为细胞模型、动物模型或临床前样本中Wnt - 5a的功能研究提供可靠数据支持，助力于靶向药物开发或疾病分子机制解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：hWNT 5A ELISA Kit; hWNT5A ELISA Kit; Protein Wnt 5a ELISA Kit; Protein Wnt-5a ELISA Kit; Protein Wnt5a ELISA Kit; Wingless type MMTV integration site family member 5A ELISA Kit; Wnt 5a ELISA Kit; WNT 5A protein ELISA Kit; WNT 5A protein precursor ELISA Kit; WNT5A ELISA Kit; WNT5A protein precursor ELISA Kit; WNT5A_HUMAN ELISA Kit
缩写：WNT5A
Uniprot No.：P41221
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 98-104 | |||||
回收率：
| The recovery of human WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 83-99 | ||||
| EDTA plasma (n=4) | 97 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected101.8391.8991.8691.68551.5711.5831.5771.3932.51.1661.2051.1861.0021.250.7320.7810.7570.5730.6250.4480.4620.4550.2710.3120.3250.3410.3330.1490.1560.2360.2470.2420.05800.1830.1850.184 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human WNT5A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled WNT5A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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