| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CI-13kA; CI13KDA; CI-13kD-A |
| Entrez GeneID | 4726 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of human NDUFS6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NDUFS6抗体的3篇示例参考文献(内容为虚构示例，仅供参考)：
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1. **文献名称**：*NDUFS6 Deficiency in Mitochondrial Complex I-Associated Encephalomyopathy*
**作者**：Smith A, et al.
**摘要**：本研究利用NDUFS6抗体对线粒体脑肌病患者的成纤维细胞进行Western blot分析，发现NDUFS6蛋白表达显著降低。通过基因测序证实了NDUFS6基因突变，提示其在复合体I组装中的关键作用，为疾病诊断提供了分子标志物。
2. **文献名称**：*Antibody-Based Detection of NDUFS6 in Colorectal Cancer Tissues*
**作者**：Zhang L, et al.
**摘要**：通过免疫组化技术结合NDUFS6抗体，研究团队发现结直肠癌组织中NDUFS6蛋白水平异常下调，且与肿瘤代谢重编程和患者预后不良相关，表明其可能作为潜在的治疗靶点。
3. **文献名称**：*Validation of a Novel NDUFS6-Specific Antibody for Mitochondrial Disease Screening*
**作者**：Johnson R, et al.
**摘要**：本研究开发了一种高特异性NDUFS6多克隆抗体，验证了其在人源细胞和小鼠模型中的灵敏度和特异性，为线粒体疾病的大规模筛查提供了可靠工具，并揭示了NDUFS6缺失与氧化应激的关系。
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**提示**：实际文献可通过PubMed、Google Scholar等平台，以关键词“NDUFS6 antibody” + “mitochondrial complex I”或相关疾病名称检索。注意筛选近5-10年研究以获取最新进展。
The NDUFS6 antibody is a research tool used to detect the NDUFS6 protein, a critical subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. Complex I facilitates NADH oxidation, proton translocation across the mitochondrial inner membrane, and ATP synthesis. NDUFS6. a nuclear-encoded Fe-S cluster-containing subunit, plays a structural and functional role in Complex I assembly and stability. Mutations in the NDUFS6 gene are linked to mitochondrial disorders, such as Leigh syndrome, cardiomyopathy, and neurodegenerative diseases, often characterized by impaired energy metabolism.
NDUFS6 antibodies are widely employed in Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and mitochondrial dysfunction in disease models. They aid in diagnosing genetic disorders and evaluating Complex I deficiencies in tissues or cell lines. These antibodies are typically raised in rabbits or mice using recombinant proteins or synthetic peptides as immunogens. Validation includes testing on knockout controls or tissues with known Complex I defects. Researchers also use NDUFS6 antibodies to explore therapeutic responses in metabolic or mitochondrial diseases, making them essential for both basic and clinical investigations into cellular bioenergetics and pathology.
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