| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HLC-1; B14.5b; NADHDH2; CI-B14.5b |
| Entrez GeneID | 4718 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human NDUFC2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NDUFC2抗体的3篇参考文献示例(注：文献为模拟虚构，仅供参考格式)：
1. **文献名称**：*NDUFC2 Antibody Validation in Mitochondrial Complex I Deficiency Disorders*
**作者**：Smith A, et al.
**摘要**：本研究验证了NDUFC2抗体在检测线粒体复合物I缺陷中的特异性，通过Western blot和免疫荧光技术，发现NDUFC2在患者肌肉组织中的表达显著降低，提示其作为复合物I功能障碍的生物标志物的潜力。
2. **文献名称**：*Role of NDUFC2 in Colorectal Cancer Progression: Insights from Immunohistochemical Analysis*
**作者**：Chen L, et al.
**摘要**：利用NDUFC2抗体对结直肠癌组织进行免疫组化分析，发现NDUFC2表达水平与肿瘤分期呈负相关，表明其在抑制线粒体代谢重编程和癌症转移中的潜在作用。
3. **文献名称**：*Development of a Novel Polyclonal Antibody for NDUFC2 and Its Application in Cardiomyopathy Models*
**作者**：Yamamoto K, et al.
**摘要**：本文报道了一种新型NDUFC2多克隆抗体的开发，并通过ELISA和免疫沉淀验证其高特异性。应用该抗体发现，心肌病小鼠模型中NDUFC2蛋白水平下降，提示其参与心脏能量代谢异常机制。
(注：实际文献需通过PubMed、Google Scholar等平台以关键词“NDUFC2 antibody”或“NDUFC2+应用领域”检索获取。)
NDUFC2 (NADH:ubiquinone oxidoreductase subunit C2) is a critical component of mitochondrial Complex I, the largest enzyme in the electron transport chain (ETC) responsible for NADH oxidation and ATP production. As a nuclear-encoded subunit, NDUFC2 localizes to the mitochondrial inner membrane and plays a structural and functional role in stabilizing Complex I assembly and facilitating proton translocation. Dysregulation of NDUFC2 has been implicated in mitochondrial disorders, neurodegenerative diseases, and cancer due to its impact on oxidative phosphorylation efficiency and cellular energy metabolism.
Antibodies targeting NDUFC2 are essential tools for studying mitochondrial biology, particularly in detecting protein expression, assessing Complex I integrity, and investigating pathological mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to evaluate NDUFC2 levels in disease models, such as Leigh syndrome, Parkinson's disease, or tumor microenvironments with metabolic reprogramming. Specific NDUFC2 antibodies also aid in distinguishing between functional and disassembled Complex I subunits in genetic or drug-induced mitochondrial dysfunction. Validation via knockout controls or siRNA knockdown is critical to ensure antibody specificity, given the high homology among ETC subunits. Research utilizing these antibodies contributes to therapeutic strategies targeting mitochondrial energy deficits in aging and metabolic diseases.
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