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产品描述：人Wnt-4蛋白(WNT4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT4含量。WNT4是重要靶点。它是Wnt蛋白家族成员，对胚胎发育、组织稳态维持等关键生理过程起重要作用。研究聚焦其信号通路机制，探索与疾病关联，如在肿瘤、生殖系统疾病等方面，有望为这些疾病治疗提供新策略。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，可广泛应用于发育生物学、肿瘤微环境研究、组织再生机制探索等科研场景，支持研究Wnt - 4在疾病模型中的表达调控及其与相关信号通路的相互作用，为细胞功能研究和分子机制解析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MGC123964 ELISA Kit; Protein Wnt-4 ELISA Kit; RP23-246F18.1 ELISA Kit; SERKAL ELISA Kit; Wingless type MMTV integration site family member 4 precursor ELISA Kit; WNT 4 ELISA Kit; WNT 4 protein precursor ELISA Kit; WNT4 ELISA Kit; WNT4_HUMAN ELISA Kit
缩写：WNT4
Uniprot No.：P56705
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of human WNT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-95 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.312.32.3052.1337501.8431.8451.8441.6723751.3311.3371.3341.162187.50.8560.8550.8560.684940.630.6210.6260.454470.4190.4140.4170.24523.50.2950.2970.2960.12400.1710.1730.172 | ||||||
货期：3-5 working days
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