| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 1/20 | Human,Mouse,Rat,Hamster |
| IHC | 咨询技术 | Human,Mouse,Rat,Hamster |
| ICC | 技术咨询 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | TXA2-R; BDPLT13 |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TBXA2R |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDUFS6抗体的3篇模拟参考文献示例，涵盖不同研究方向和实验方法：
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1. **文献名称**：*Mitochondrial Complex I Deficiency Caused by NDUFS6 Mutations in Patients with Leigh Syndrome*
**作者**：Smith J, et al.
**摘要**：本研究通过Western blot和免疫荧光技术，利用NDUFS6抗体分析患者成纤维细胞中线粒体复合物I的表达。结果显示，NDUFS6蛋白水平显著降低，表明其突变导致复合物I组装缺陷，与Leigh综合征的病理机制相关。
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2. **文献名称**：*Expression Analysis of Mitochondrial Complex I Subunits in Colorectal Cancer*
**作者**：Zhang L, et al.
**摘要**：采用NDUFS6抗体对结直肠癌组织进行免疫组化分析，发现肿瘤组织中NDUFS6表达显著下调，且与患者生存率呈正相关。研究提示NDUFS6可能作为线粒体代谢异常的潜在生物标志物。
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3. **文献名称**：*Functional Characterization of NDUFS6 Knockout Mice Using Antibody-Based Proteomic Approaches*
**作者**：Brown R, et al.
**摘要**：通过构建NDUFS6基因敲除小鼠模型，结合NDUFS6抗体的Western blot和免疫电镜技术，证实NDUFS6缺失导致线粒体复合物I功能丧失，并引发氧化磷酸化障碍及心肌病变。
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(注：以上文献为模拟内容，实际引用需根据真实数据库检索结果调整。)
The NDUFS6 antibody is a crucial tool for studying the NDUFS6 protein, a core subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. Complex I, the largest respiratory complex, plays a vital role in ATP synthesis by facilitating electron transfer from NADH to ubiquinone, coupled with proton translocation. NDUFS6 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 6) is essential for Complex I assembly, stability, and catalytic activity. Mutations in the NDUFS6 gene are linked to mitochondrial disorders, such as Leigh syndrome, and neurodegenerative diseases, making this protein a focus in mitochondrial research.
NDUFS6 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and alterations in disease models. They help investigate mitochondrial dysfunction in conditions like cancer, Parkinson’s disease, and metabolic syndromes. Commercially available NDUFS6 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins as immunogens. Validation includes testing specificity via knockout cell lines or siRNA-mediated knockdown. Researchers prioritize antibodies with high specificity and cross-reactivity across species (e.g., human, mouse, rat) to ensure reproducibility. As mitochondrial research expands, NDUFS6 antibodies remain pivotal for unraveling mechanisms of energy metabolism and disease pathogenesis.
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