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产品描述：小鼠Wnt-4蛋白(WNT4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT4含量。WNT4是一个重要靶点。它属于Wnt蛋白家族，在胚胎发育、组织稳态维持等多方面起关键作用。研究机制主要围绕WNT信号通路展开，其异常激活或抑制与多种疾病发生发展相关，如肿瘤、生殖系统疾病等，是潜在的药物作用靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，支持科研人员探究Wnt - 4在细胞信号转导、发育生物学或病理机制中的功能；Wnt - 4蛋白表达水平变化常作为相关疾病模型研究的分子标志物本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt4 ELISA kit; Wnt-4Protein Wnt-4 ELISA kit
缩写：WNT4
Uniprot No.：P22724
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-105 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-91 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of mouse WNT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5001.8151.8001.8081.6752501.2111.3011.2561.1231250.7670.7980.7830.65062.50.4850.4680.4770.34431.20.3010.3120.3070.17415.60.2180.2090.2140.0817.80.1770.1800.1790.04600.1320.1340.133 | |||||||
货期：3-5 working days
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