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产品描述：人Wnt-3a蛋白(WNT3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的WNT3A含量。WNT3A是一种重要的靶点。它是Wnt信号通路的关键配体，在胚胎发育、组织稳态维持等过程中发挥重要作用。研究聚焦于其在肿瘤、神经系统疾病等病理机制中的作用，探索通过调控WNT3A来干预相关信号通路，为疾病治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础研究领域如干细胞定向分化机制探索、肿瘤微环境调控分析或药物开发中Wnt通路活性评估等科研场景，为体外实验提供高效稳定的检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Protein Wnt 3a Precursor ELISA Kit; Protein Wnt-3a ELISA Kit; Wingless type MMTV integration site family member 3A ELISA Kit; Wnt 3a ELISA Kit; wnt3a ELISA Kit; WNT3A protein ELISA Kit; WNT3A_HUMAN ELISA Kit
缩写：WNT3A
Uniprot No.：P56704
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-98 | ||||
| EDTA plasma (n=4) | 96 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.0301.9521.9911.823 51.5491.4761.5131.345 2.51.0921.0141.0530.885 1.250.7120.7090.7110.543 0.6250.4870.4490.4680.300 0.3120.3720.3590.3660.198 0.1560.2730.2540.2640.096 00.1700.1660.168 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human WNT3A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled WNT3A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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