| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2 |
| Entrez GeneID | 140739 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human NCE2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于NCE2(钠钙交换体2)抗体的虚构参考文献示例(内容为模拟创作，非真实文献)：
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1. **文献名称**: "Characterization of a Novel NCE2-Specific Antibody for Cardiovascular Research"
**作者**: Smith, J.R. et al.
**摘要**: 本研究开发了一种高特异性兔源多克隆抗体，靶向钠钙交换体2(NCE2)的胞外结构域。通过免疫印迹和免疫组化验证，该抗体能有效识别心脏组织中的NCE2蛋白，并成功应用于心力衰竭模型中的蛋白表达水平分析，为心血管疾病机制研究提供工具。
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2. **文献名称**: "NCE2 Antibody-Based Detection of Tumor Metastasis Markers"
**作者**: Chen, L. & Wang, H.
**摘要**: 文章报道了一种单克隆NCE2抗体在癌症研究中的应用。通过流式细胞术和免疫荧光证实，该抗体可特异性结合乳腺癌细胞表面NCE2蛋白，并发现其表达水平与肿瘤转移潜力呈正相关，为癌症诊断提供潜在生物标志物。
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3. **文献名称**: "Cross-Species Reactivity of Anti-NCE2 Antibodies in Neurological Disorders"
**作者**: Gonzalez, M. et al.
**摘要**: 研究评估了商业NCE2抗体在小鼠、大鼠及人类脑组织中的交叉反应性。结果显示，部分抗体存在非特异性结合问题，但经优化后能准确检测阿尔茨海默病模型中NCE2的异常分布，强调抗体验证在神经科学研究中的重要性。
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(注：以上文献为示例性内容，实际研究中需以真实数据库检索结果为准。)
NCE2 antibodies target the Na+/Ca²⁺ exchanger 2 (NCX2), a membrane protein encoded by the *SLC8A2* gene. NCX2 belongs to the solute carrier family 8 (SLC8) and plays a critical role in cellular calcium homeostasis by mediating the bidirectional exchange of Na⁺ and Ca²⁺ across the plasma membrane. Predominantly expressed in the brain, particularly in neurons, NCX2 regulates intracellular Ca²⁺ levels during neuronal signaling and helps maintain synaptic plasticity. Dysregulation of NCX2 has been implicated in neurological disorders, including epilepsy, stroke, and neurodegenerative diseases.
NCE2 antibodies are widely used in research to study NCX2 expression, localization, and function in both physiological and pathological contexts. These antibodies enable techniques like immunohistochemistry, Western blotting, and immunofluorescence, aiding in the visualization of NCX2 distribution in tissues or cultured cells. Additionally, they are employed to investigate NCX2 interactions with other proteins or signaling pathways. As calcium dyshomeostasis is a hallmark of many neurological conditions, NCE2 antibodies serve as essential tools for exploring NCX2’s role in disease mechanisms and potential therapeutic targets. Their specificity and reliability are validated through knockout controls or peptide blocking assays to ensure accurate experimental outcomes.
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