首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人原癌基因Wnt-3蛋白(WNT3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WNT3含量。WNT3 是一种重要的靶点。其属于 Wnt 蛋白家族，在胚胎发育、组织稳态维持等多个生理过程中发挥关键作用。研究机制上，它主要通过与受体结合激活 Wnt 信号通路，异常激活或抑制该通路与肿瘤、神经系统疾病等多种病症相关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，的应用场景涵盖肿瘤生物学研究中的Wnt通路激活状态评估、干细胞分化模型中信号分子动态监测，以及疾病动物模型的组织特异性表达分析等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Int 4 ELISA Kit; INT4 ELISA Kit; MGC131950 ELISA Kit; MGC138321 ELISA Kit; MGC138323 ELISA Kit; Proto-oncogene Int-4 homolog ELISA Kit; Proto-oncogene Wnt-3 ELISA Kit; wingless type MMTV integration site family member 3 ELISA Kit; Wnt 3 proto oncogene protein ELISA Kit; WNT 3 proto oncogene protein precursor ELISA Kit; wnt3 ELISA Kit; WNT3_HUMAN ELISA Kit
缩写：WNT3
Uniprot No.：P56703
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human WNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 98 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2462.3012.2742.05851.7051.6421.6741.4582.51.2441.3201.2821.0661.250.9050.8730.8890.6730.6250.5410.5420.5420.3260.3120.4350.4670.4510.2350.1560.3120.3250.3190.10300.2150.2160.216 | ||||||
货期：3-5 working days
×
