首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠原癌基因Wnt-3蛋白(WNT3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WNT3含量。WNT3作为一种靶点，属于Wnt蛋白家族。它在胚胎发育、细胞增殖和分化等过程中发挥重要作用。其异常表达与肿瘤发生、发展密切相关。研究主要聚焦于其信号通路机制，旨在通过干预该靶点调控相关信号，为疾病治疗提供新策略。试剂盒检测范围为39.07 pg/mL-2500 pg/mL，该产品可广泛应用于基础研究领域，包括Wnt信号通路调控机制解析、基因功能验证、药物靶点筛选及疾病动物模型构建等科研场景，为探索WNT3在细胞增殖分化、肿瘤微环境调控及再生医学研究中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt3 ELISA Kit; Int-4 ELISA Kit; Wnt-3 ELISA Kit; Proto-oncogene Wnt-3 ELISA Kit; Proto-oncogene Int-4 ELISA Kit
缩写：WNT3
Uniprot No.：P17553
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/ml-2500 pg/ml
灵敏度：9.75 pg/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 86 | ||
| Range % | 82-91 | |||
| 1:2 | Average % | 101 | ||
| Range % | 98-105 | |||
| 1:4 | Average % | 87 | ||
| Range % | 84-91 | |||
| 1:8 | Average % | 100 | ||
| Range % | 95-105 |
回收率：
| The recovery of mouse WNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 25002.5472.6012.5742.411 12502.2912.2002.2462.083 6251.5431.6011.5721.409 312.50.9710.9680.9700.807 156.250.5720.6010.5870.424 78.130.4140.4000.4070.244 39.070.2950.2900.2930.130 00.1610.1640.163 | |||||||
货期：3-5 working days
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