| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NCK2; GRB4; Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta |
| Entrez GeneID | 8440 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Nck beta |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NCK2抗体的示例性参考文献(内容为模拟，非真实文献)：
1. **"NCK2 modulates EGFR signaling in lung cancer progression"**
- 作者：Li, X. et al.
- 摘要：研究通过Western blot和免疫共沉淀技术，利用NCK2抗体揭示其在肺癌细胞中与EGFR的相互作用，表明NCK2过表达促进肿瘤侵袭和化疗耐药。
2. **"NCK2 regulates synaptic plasticity via dendritic spine formation"**
- 作者：Smith, J. & Tanaka, H.
- 摘要：通过免疫组化和基因敲除模型，结合NCK2抗体的定位分析，发现NCK2在小鼠海马神经元中调控突触可塑性，影响学习和记忆功能。
3. **"A novel role of NCK2 in TGF-β signaling pathway"**
- 作者：Garcia, R. et al.
- 摘要：利用NCK2特异性抗体阻断实验，证明NCK2在成纤维细胞中介导TGF-β信号传导，影响细胞迁移和纤维化疾病进程。
4. **"Development of a high-affinity monoclonal NCK2 antibody for diagnostic applications"**
- 作者：Wang, Y. et al.
- 摘要：报道一种新型NCK2单克隆抗体的开发，验证其在乳腺癌组织芯片中的灵敏度和特异性，提示其作为潜在生物标志物的价值。
(注：以上文献为示例性内容，实际研究中请通过PubMed或Google Scholar检索真实文献。)
The NCK2 antibody is a crucial tool in studying the role of NCK2 (Non-catalytic region of tyrosine kinase adaptor protein 2), a cytosolic adaptor protein involved in intracellular signaling pathways. NCK2. also known as GRB4. shares structural homology with NCK1 and contains three SH3 domains and one SH2 domain, enabling interactions with phosphorylated tyrosine residues on receptor tyrosine kinases (RTKs) and other signaling molecules. It regulates processes like cell migration, cytoskeletal reorganization, and growth factor signaling by linking activated receptors to downstream effectors such as Ras and Rho GTPases. NCK2 dysfunction has been implicated in cancer, developmental disorders, and neurological conditions.
NCK2 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect NCK2 expression, localization, and protein-protein interactions. These antibodies are typically validated for specificity through knockout controls or siRNA knockdown to minimize cross-reactivity with NCK1. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary depending on commercial sources. Researchers rely on NCK2 antibodies to explore its role in RTK-driven pathways, tumor metastasis, and neuronal guidance, making them essential for both basic and translational studies in cell signaling and disease mechanisms.
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