| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAPH2 |
| Entrez GeneID | 29781 |
| WB Predicted band size | Calculated MW: 68 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NCAPH2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NCAPH2抗体的3篇参考文献的简要总结(注：以下为模拟文献，实际引用需核实)：
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1. **文献名称**：*NCAPH2 regulates meiotic chromosome structure and homolog pairing in mammalian oocytes*
**作者**：Wang et al., 2021
**摘要**：本研究通过免疫荧光染色结合NCAPH2抗体，揭示了NCAPH2在哺乳动物卵母细胞减数分裂过程中对染色体凝缩和同源染色体配对的关键作用。抗体特异性验证显示其适用于体内外蛋白定位分析。
2. **文献名称**：*Condensin II subunit NCAPH2 modulates DNA repair in cancer cells*
**作者**：Li et al., 2019
**摘要**：利用NCAPH2抗体进行Western blot和免疫沉淀实验，作者发现NCAPH2通过维持基因组稳定性参与DNA损伤修复通路，其表达缺失导致癌细胞对辐射敏感性增加。
3. **文献名称**：*A functional screen identifies NCAPH2 as a driver of prostate cancer progression*
**作者**：Chen et al., 2020
**摘要**：该研究通过免疫组化(IHC)结合NCAPH2抗体，证实NCAPH2在前列腺癌组织中高表达，且与患者预后不良相关。抗体特异性验证支持其在临床样本中的可靠应用。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“NCAPH2 antibody”、“condensin II NCAPH2”进一步检索。
The NCAPH2 antibody targets the non-SMC condensin II complex subunit H2 (NCAPH2), a key component of the condensin II complex involved in chromosome condensation and mitotic progression. NCAPH2. also known as kleisin β, plays a critical role in organizing chromatin architecture during cell division by facilitating the structural remodeling of chromosomes. This protein ensures proper sister chromatid resolution and prevents chromosomal entanglements, contributing to genome stability. NCAPH2 is distinct from its paralog NCAPH (condensin I subunit) in its nuclear localization during interphase and specific regulatory roles in condensin II-mediated chromatin compaction.
Antibodies against NCAPH2 are widely used to study chromosome dynamics, mitotic regulation, and DNA repair mechanisms. They enable researchers to detect NCAPH2 expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Dysregulation of NCAPH2 has been linked to genomic instability disorders and cancer, making its antibody a valuable tool for investigating disease mechanisms. Recent studies also explore its role in meiosis and developmental biology. Validation of NCAPH2 antibodies typically includes testing in knockout cell lines or tissues to confirm specificity. These reagents support both basic research on chromosome biology and translational studies targeting mitotic vulnerabilities in cancer therapy.
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