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产品描述：人Wnt-2b蛋白(WNT2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WNT2B含量。WNT2B是一个值得关注的靶点。它属于WNT蛋白家族成员，在胚胎发育、细胞增殖与分化等过程中发挥重要作用。研究聚焦于其信号通路机制，探索其在肿瘤发生发展、组织再生修复等方面的作用，有望为相关疾病治疗提供新思路。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本试剂盒可灵敏检测天然或重组蛋白浓度，配套预包被特异性捕获抗体、生物素标记检测抗体及链霉亲和素 - HRP系统，提供标准化操作流程和预混试剂，支持科研人员开展发育生物学研究、肿瘤微环境分析、干细胞定向分化模型构建等基础实验。该产品兼容常规酶标仪检测，适用于体外培养细胞、动物模型组织或生物液体样本的靶蛋白表达水平分析，为探索Wnt信号网络调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：WNT2B; WNT13; Protein Wnt-2b; Protein Wnt-13
缩写：WNT2B
Uniprot No.：Q93097
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 83-94 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-101 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-105 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-101 | ||||||
回收率：
| The recovery of human WNT2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-105 | |||||
| EDTA plasma (n=4) | 104 | 88-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 40002.5132.3552.4342.285 20002.0121.9801.9961.847 10001.2201.2221.2211.072 5000.7250.7210.7230.574 2500.4170.4210.4190.270 1250.2980.2820.2900.141 62.50.2270.2240.2260.077 00.1480.1500.149 | |||||||
货期：3-5 working days
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