首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Wnt-2蛋白(WNT2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT2含量。WNT2 是一种重要靶点。它是 Wnt 信号通路成员，在胚胎发育、细胞增殖与分化等过程中发挥关键作用。异常激活会促进肿瘤发生、发展。科研聚焦其在信号传导中的分子机制，探索以它为靶点开发癌症等疾病的新型治疗方法。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于多种实验场景，如研究发育生物学中Wnt信号通路的激活机制、肿瘤微环境中WNT2的表达调控，或评估基因编辑模型小鼠体内蛋白水平变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt2 ELISA Kit; Irp ELISA Kit; Wnt-2 ELISA Kit; Protein Wnt-2 ELISA Kit; INT-1-related protein ELISA Kit; IRP ELISA Kit
缩写：WNT2
Uniprot No.：P21552
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 83-87 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-108 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of mouse WNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-101 | ||||
| EDTA plasma (n=4) | 89 | 83-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3152.5012.4082.2475001.4781.5891.5341.3732500.9280.9840.9560.7951250.6030.5870.5950.43462.50.3250.3400.3330.17231.250.2570.2770.2670.10615.60.2010.2150.2080.04700.1570.1640.161 | ||||||
货期：3-5 working days
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