首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Wnt-16蛋白(WNT16)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WNT16含量。小鼠Wnt-16蛋白（WNT16）在骨骼稳态中起关键作用，其功能缺失会导致骨密度降低和骨折风险增加。WNT16通过调控成骨细胞功能影响骨骼发育，其调控机制涉及内含子2区域的启动子活性与CPED1增强子区域的物理互作，可能影响骨骼稳态及骨质疏松症治疗。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于小鼠模型或细胞实验中Wnt - 16蛋白表达水平的功能研究，例如探索骨骼发育异常机制、肿瘤微环境信号传导或干细胞定向分化调控等科研场景；适用于基础研究与药物开发中Wnt信号通路的分子机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt16 ELISA Kit; Protein Wnt-16 ELISA Kit
缩写：WNT16
Uniprot No.：Q9QYS1
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 101-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 106 | 101-111 | ||||
| EDTA plasma (n=4) | 92 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.3792.4652.4222.26820002.0221.9701.9961.84210001.4501.5601.5051.3515000.8650.8990.8820.7282500.5310.5270.5290.3751250.3750.3830.3790.22562.50.2880.2780.2830.12900.1580.1500.154 | ||||||
货期：3-5 working days
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