| WB | 1/500-1/1000 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 1/50-1/100 | Mouse,Rat |
| ICC | 1/50-1/200 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | Myeloperoxidase; MPO |
| Entrez GeneID | 4353 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | Recombinant protein of human Myeloperoxidase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Myeloperoxidase(MPO)抗体的代表性文献概览：
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1. **文献名称**: *Anti-neutrophil cytoplasmic autoantibodies induce neutrophils to degranulate and produce oxygen radicals in vitro*
**作者**: Falk RJ, Jennette JC
**摘要**: 该研究首次证实抗中性粒细胞胞浆抗体(ANCA)中的MPO抗体可直接激活中性粒细胞，导致脱颗粒和活性氧释放，提示其在血管炎发病机制中的直接作用。
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2. **文献名称**: *Pathogenesis of ANCA-associated vasculitis: new possibilities for intervention*
**作者**: Kallenberg CGM
**摘要**: 综述文章系统分析了MPO抗体与PR3抗体的差异，指出MPO抗体阳性患者更易出现肾脏受累，并讨论了靶向补体通路的新型治疗策略。
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3. **文献名称**: *Neutrophil extracellular traps (NETs) in autoimmune diseases*
**作者**: Radic M et al.
**摘要**: 研究发现MPO抗体可通过干扰NETs(中性粒细胞胞外诱捕网)的清除，加剧自身抗原暴露，从而促进ANCA相关性血管炎的慢性炎症过程。
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4. **文献名称**: *Experimental models of anti-myeloperoxidase antibody-associated vasculitis*
**作者**: Xiao H et al.
**摘要**: 通过小鼠模型证明MPO抗体可直接诱发坏死性肾小球肾炎和肺出血，为抗体致病性提供了直接实验证据，并揭示Fc受体信号在此过程中的关键作用。
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这些文献涵盖了MPO抗体的致病机制、临床关联及实验模型研究，反映其在自身免疫性疾病中的核心地位。
Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme primarily found in the azurophilic granules of neutrophils and lysosomes of monocytes. It plays a critical role in the innate immune response by catalyzing the production of hypochlorous acid (HOCl) from hydrogen peroxide and chloride ions, which contributes to microbial killing. MPO antibodies, predominantly of the IgG class, target this enzyme and are clinically significant in autoimmune disorders, particularly antineutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV), such as microscopic polyangiitis (MPA) and granulomatosis with polyangiitis (GPA).
These autoantibodies are detected via immunoassays (e.g., ELISA) or indirect immunofluorescence (IIF). Unlike proteinase 3 (PR3) antibodies, which show cytoplasmic staining (c-ANCA), MPO antibodies typically produce a perinuclear pattern (p-ANCA). Their presence aids in diagnosing and classifying AAV, correlating with disease activity and organ involvement, especially in renal-limited vasculitis or pulmonary-renal syndromes.
MPO antibodies are also implicated in drug-induced vasculitis and other autoimmune conditions, such as lupus or eosinophilic granulomatosis with polyangiitis (EGPA). Research continues to explore their pathogenic role, including neutrophil activation and endothelial damage mechanisms. Clinically, monitoring MPO antibody titers assists in guiding immunosuppressive therapy and predicting relapses, underscoring their diagnostic and prognostic utility.
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